Novus Biologicals™ Breast Matched Tumor and Normal Tissue Lysate

Description

• These lysates have not been subjected to denaturing or reducing conditions
• This allows the tissue or cell lysate to be used in a variety of applications; to study protein-protein interaction, ligand binding, ELISA, immunoprecipitation, 1D and 2D gel electrophoresis, and Western blotting for the detection of specific protein targets
• For use in 1D and 2D gel electrophoresis, the addition of a denaturing gel loading buffer with reducing agents may be required
• Buffer requirements for performing protein-protein interaction and ligand binding studies can vary significantly from RIPA buffer and may require modifications
• In most cases, tissue lysates in RIPA buffer can be used, directly in standard ELISA and immunoprecipitation assays
• These lysates are proteomic discovery tools
• Researchers should validate and optimize for individual use
• The Breast Matched Tumor & Normal Tissue Lysate is derived from human.