Novus Biologicals™ Lightning-Link (R) PE-Cy5 Antibody Labeling Kit

Description

• PE/Cy5 is a tandem conjugate
• The PE is excited at 488nm and functions as an energy donor for the Cy5
• The energy is transferred via FRET (Fluorescence Resonance Energy Transfer) to the Cy5, which emits then the energy received in the form of long wavelength light at 670nm
• The antibody is covalently bonded to the label in a directional and controlled process at near-neutral pH
• In view of the large size of the PE-Cy5 label, the amount of antibody used in a labeling reaction with the tandem must always be less than the pack size of LL PE-Cy5, in order that the PE-Cy5 is in a slight molar excess
• The best ratio for any new antibody reagent must be determined by experimentation but 50-60ug of IgG antibody for every 100ug of LL PE-Cy5 usually gives optimal results
• 50ug of antibody corresponds to an Ab:PE-Cy5 molar ratio of 1:1
• The amount of antibody used for labeling should be the same as the pack size of LL-PE-Cy5
• For any new antibody, optimization of the ratio of antibody to dye is often worthwhile
• If your antibody needs to be concentrated, purified, or cleaned up, consider purchasing an antibody clean up kit.