PI16146

Thermo Scientific™ pMCS-Gaussia Luc Vector for Luciferase Assays

Manufacturer: Thermo Scientific™

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Pack Size SKU Availability Price
Each of 1 PI16146-Each-of-1 In Stock ₹ 12,859.67

PI16146 - Each of 1

₹ 12,859.67

In Stock

Quantity

1

Base Price: ₹ 12,859.67

GST (18%): ₹ 2,314.741

Total Price: ₹ 15,174.411

Description

pMCS-Gaussia Luc Vector

Cloning Method

Restriction Enzyme/MCS

Quantity

10 μg

Reporter Gene

Gaussia Luciferase

Product Line

Pierce™

Type

Luc Vector

Delivery Type

Transfection

Content And Storage

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C.

Selection Agent (Eukaryotic)

Puromycin

For Use With (Application)

Reporter Assays

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Description

  • The Thermo Scientific™ pMCS-Gaussia Luc Vector is a multiple cloning site plasmid designed to accept a promoter sequence for study of gene regulation using the naturally secreting Gaussia luciferase reporter
  • The Gaussia Luc Vectors contain a gene cloned from the copepod, Gaussia princeps
  • The gene encodes a naturally-secreted bioluminescent Gaussia luciferase (approx
  • 20kDa), which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates
  • The pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential
  • The pCMV and pTK vectors have the luciferase gene under the CMV (Cytomegalovirus) promoter and Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter, respectively
  • These constitutive expression vectors can be used as normalization controls to account for experimental variation in combination with other reporters
  • Highlights: Naturally-secreting Gaussia luciferase gene, optimized for high expression in mammalian systems Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively High-copy pUC bacterial DNA replication origin Two control vectors available with strong (CMV) or weak (TK) constitutive promoters for co-transfection and normalization

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Thermo Scientific™

PI16146

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Description:
pMCS-Gaussia Luc Vector

Cloning Method:
Restriction Enzyme/MCS

Quantity:
10 μg

Reporter Gene:
Gaussia Luciferase

Product Line:
Pierce™

Type:
Luc Vector

Delivery Type:
Transfection

Content And Storage:
Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C.

Selection Agent (Eukaryotic):
Puromycin

For Use With (Application):
Reporter Assays

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Description:
pCMV-Gaussia Luc Vector

Cloning Method:
Restriction Enzyme/MCS

Quantity:
10 μg

Reporter Gene:
Gaussia Luciferase

Product Line:
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Type:
Luc Vector

Delivery Type:
Transfection

Content And Storage:
Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C.

Selection Agent (Eukaryotic):
Puromycin

For Use With (Application):
Reporter Assays

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Description:
pMCS-Cypridina Luc Vector

Cloning Method:
Restriction Enzyme/MCS

Quantity:
10 μg

Reporter Gene:
Cypridina Luciferase

Product Line:
Pierce™

Type:
Luc Vector

Delivery Type:
Transfection

Content And Storage:
Store between -70°C and -20°C.

Selection Agent (Eukaryotic):
Puromycin

For Use With (Application):
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Description:
pCMV-Cypridina Luc Vector

Cloning Method:
Restriction Enzyme/MCS

Quantity:
10 μg

Reporter Gene:
Cypridina Luciferase

Product Line:
Pierce™

Type:
Luc Vector

Delivery Type:
Transfection

Content And Storage:
Store between -70°C and -20°C.

Selection Agent (Eukaryotic):
Puromycin

For Use With (Application):
Reporter Assays