PI16192
Thermo Scientific™ pTK-Gaussia-Dura Luc Vector for Luciferase Assays
Manufacturer: Thermo Scientific™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI16192-Each-of-1 | In Stock | ₹ 12,637.21 |
PI16192 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 12,637.21
GST (18%): ₹ 2,274.698
Total Price: ₹ 14,911.908
Description
pTK-Gaussia-Dura Luc Vector
Cloning Method
Restriction Enzyme/MCS
Quantity
10 μg
Reporter Gene
Gaussia Dura Luciferase
For Use With (Application)
Reporter Assays
Type
Luc Vector
Delivery Type
Transfection
Content And Storage
Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C.
Selection Agent (Eukaryotic)
Puromycin
Promoter
TK
Related Products
Description
- Thermo Scientific™ pMCSGaussia-Dura Luc Vector is a multiple cloning site plasmid designed to accept a promoter sequence for glow-analysis of gene regulation using the naturally secreting Gaussia luciferase reporter
- The Gaussia Dura Luc Vectors contains a mutant form of the Gaussia luciferase gene that confers better bioluminescent signal stability than native luciferase
- Gaussia -Dura luciferase (approx
- 20kDa) is a secreted protein, which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates
- The pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential
- The pCMV and pTK vectors have the luciferase gene under the CMV (Cytomegalovirus) promoter and Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter, respectively
- These constitutive expression vectors can be used as normalization controls to account for experimental variation in combination with other reporters
- Highlights: Naturally-secreting Gaussia -Dura luciferase gene, optimized for high expression and glow-stability in mammalian systems Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively High-copy pUC bacterial DNA replication origin Two control vectors available with strong (CMV) or weak (TK) constitutive promoters for co-transfection and normalization
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Description: pTK-Gaussia-Dura Luc Vector
Cloning Method: Restriction Enzyme/MCS
Quantity: 10 μg
Reporter Gene: Gaussia Dura Luciferase
For Use With (Application): Reporter Assays
Type: Luc Vector
Delivery Type: Transfection
Content And Storage: Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C.
Selection Agent (Eukaryotic): Puromycin
Promoter: TK
Description:
pTK-Gaussia-Dura Luc Vector
Cloning Method:
Restriction Enzyme/MCS
Quantity:
10 μg
Reporter Gene:
Gaussia Dura Luciferase
For Use With (Application):
Reporter Assays
Type:
Luc Vector
Delivery Type:
Transfection
Content And Storage:
Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C.
Selection Agent (Eukaryotic):
Puromycin
Promoter:
TK
Description: __
Cloning Method: __
Quantity: 100 Reaction Kit
Reporter Gene: __
For Use With (Application): __
Type: Assay Kit
Delivery Type: __
Content And Storage: • Firefly Luc One-Step Substrate, Lyophilized (makes 1μL of 1X), store at -20°C• Firefly Luc One-Step Assay Buffer, 11 mL, store at -20°CUpon receipt store kit at -20°C.
Selection Agent (Eukaryotic): __
Promoter: __
Description:
__
Cloning Method:
__
Quantity:
100 Reaction Kit
Reporter Gene:
__
For Use With (Application):
__
Type:
Assay Kit
Delivery Type:
__
Content And Storage:
• Firefly Luc One-Step Substrate, Lyophilized (makes 1μL of 1X), store at -20°C• Firefly Luc One-Step Assay Buffer, 11 mL, store at -20°CUpon receipt store kit at -20°C.
Selection Agent (Eukaryotic):
__
Promoter:
__
Description: __
Cloning Method: __
Quantity: 1000 Reaction Kit
Reporter Gene: __
For Use With (Application): __
Type: Assay Kit
Delivery Type: __
Content And Storage: • Firefly Luc One-Step Substrate, Lyophilized (makes 1 mL of 1X), store at -20°C• Firefly Luc One-Step Assay Buffer, 115 mL, store at -20°CUpon receipt store kit at -20°C.
Selection Agent (Eukaryotic): __
Promoter: __
Description:
__
Cloning Method:
__
Quantity:
1000 Reaction Kit
Reporter Gene:
__
For Use With (Application):
__
Type:
Assay Kit
Delivery Type:
__
Content And Storage:
• Firefly Luc One-Step Substrate, Lyophilized (makes 1 mL of 1X), store at -20°C• Firefly Luc One-Step Assay Buffer, 115 mL, store at -20°CUpon receipt store kit at -20°C.
Selection Agent (Eukaryotic):
__
Promoter:
__
Description: __
Cloning Method: __
Quantity: 10 Reactions
Reporter Gene: __
For Use With (Application): __
Type: __
Delivery Type: __
Content And Storage: Sufficient For: 10 biotin-labeling reactions (each with 100 ng DNA)• Heptanucleotide Mix (5X), 100 μL• Deoxynucleotide Mix (5X), 80 μL• Reaction Buffer (10X), 50 μL• DNA Polymerase Klenow Fragment (5U/μL), 10 μL• Control DNA (250 ng/μL), 5 μL• Ammonium Acetate (5M), 1 mL• EDTA (0.5M, pH 8.0), 1 mL• Nuclease-free Water, 1 mL• Biotin-11-dUTP, 20 μLStore all components at -20°C.
Selection Agent (Eukaryotic): __
Promoter: __
Description:
__
Cloning Method:
__
Quantity:
10 Reactions
Reporter Gene:
__
For Use With (Application):
__
Type:
__
Delivery Type:
__
Content And Storage:
Sufficient For: 10 biotin-labeling reactions (each with 100 ng DNA)• Heptanucleotide Mix (5X), 100 μL• Deoxynucleotide Mix (5X), 80 μL• Reaction Buffer (10X), 50 μL• DNA Polymerase Klenow Fragment (5U/μL), 10 μL• Control DNA (250 ng/μL), 5 μL• Ammonium Acetate (5M), 1 mL• EDTA (0.5M, pH 8.0), 1 mL• Nuclease-free Water, 1 mL• Biotin-11-dUTP, 20 μLStore all components at -20°C.
Selection Agent (Eukaryotic):
__
Promoter:
__