PI16157
Thermo Scientific™ pTK-Red Firefly Luc Vector for Luciferase Assays
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI16157-Each-of-1 | In Stock | ₹ 13,381.58 |
PI16157 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 13,381.58
GST (18%): ₹ 2,408.684
Total Price: ₹ 15,790.264
Description
pTK-Red Firefly Luc Vector
Cloning Method
Restriction Enzyme ⁄ MCS
Quantity
10 μg
Reporter Gene
Red Firefly Luciferase
For Use With (Application)
Reporter Assays
Type
Luc Vector
Delivery Type
Transfection
Content And Storage
Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C.
Selection Agent (Eukaryotic)
Puromycin
Promoter
TK
Related Products
Description
- The Thermo Scientific™ pMCS-Red Firefly Luc Vector is a multiple cloning site plasmid designed to accept a promoter sequence for study of gene regulation using the intracellular red firefly luciferase reporter
- The Red Firefly Luc Vectors contain a mutant form of the firefly luciferase gene that has a red-shifted emission spectrum
- The pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential
- The pCMV and pTK vectors have the luciferase gene under the CMV (Cytomegalovirus) promoter and Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter, respectively
- These constitutive expression vectors can be used as normalization controls to account for experimental variation in combination with other reporters
- Highlights: Intracellular Red Firefly Luciferase gene, optimized for high expression in mammalian systems Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively High-copy pUC bacterial DNA replication origin Two control vectors available with strong (CMV) or weak (TK) constitutive promoters for co-transfection and normalization
Compare Similar Items
Show Difference
Description: pTK-Red Firefly Luc Vector
Cloning Method: Restriction Enzyme ⁄ MCS
Quantity: 10 μg
Reporter Gene: Red Firefly Luciferase
For Use With (Application): Reporter Assays
Type: Luc Vector
Delivery Type: Transfection
Content And Storage: Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C.
Selection Agent (Eukaryotic): Puromycin
Promoter: TK
Description:
pTK-Red Firefly Luc Vector
Cloning Method:
Restriction Enzyme ⁄ MCS
Quantity:
10 μg
Reporter Gene:
Red Firefly Luciferase
For Use With (Application):
Reporter Assays
Type:
Luc Vector
Delivery Type:
Transfection
Content And Storage:
Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C.
Selection Agent (Eukaryotic):
Puromycin
Promoter:
TK
Description: __
Cloning Method: __
Quantity: 100 Reaction Kit
Reporter Gene: __
For Use With (Application): __
Type: Assay Kit
Delivery Type: __
Content And Storage: Sufficient For: 100 luciferase assays in microplate wells• Gaussia Flash Assay Buffer, 5 mL (store at 4°C)• 100X Coelenterazine, 50 μL (store at -80°C)• Luciferase Cell Lysis Buffer (2X), 6 mL (store at room temperature)Kit may be stored at -80°C, or individually at indicated temperatures.
Selection Agent (Eukaryotic): __
Promoter: __
Description:
__
Cloning Method:
__
Quantity:
100 Reaction Kit
Reporter Gene:
__
For Use With (Application):
__
Type:
Assay Kit
Delivery Type:
__
Content And Storage:
Sufficient For: 100 luciferase assays in microplate wells• Gaussia Flash Assay Buffer, 5 mL (store at 4°C)• 100X Coelenterazine, 50 μL (store at -80°C)• Luciferase Cell Lysis Buffer (2X), 6 mL (store at room temperature)Kit may be stored at -80°C, or individually at indicated temperatures.
Selection Agent (Eukaryotic):
__
Promoter:
__
Description: __
Cloning Method: __
Quantity: 1000 Reaction Kit
Reporter Gene: __
For Use With (Application): __
Type: Assay Kit
Delivery Type: __
Content And Storage: Sufficient For: 1000 luciferase assays in microplate wells• Gaussia Flash Assay Buffer, 50 mL (store at 4°C)• 100X Coelenterazine, 0.5 mL (store at -80°C)• Luciferase Cell Lysis Buffer (2X), 60 mL (store at room temperature)Kit may be stored at -80°C, or individually at indicated temperatures.
Selection Agent (Eukaryotic): __
Promoter: __
Description:
__
Cloning Method:
__
Quantity:
1000 Reaction Kit
Reporter Gene:
__
For Use With (Application):
__
Type:
Assay Kit
Delivery Type:
__
Content And Storage:
Sufficient For: 1000 luciferase assays in microplate wells• Gaussia Flash Assay Buffer, 50 mL (store at 4°C)• 100X Coelenterazine, 0.5 mL (store at -80°C)• Luciferase Cell Lysis Buffer (2X), 60 mL (store at room temperature)Kit may be stored at -80°C, or individually at indicated temperatures.
Selection Agent (Eukaryotic):
__
Promoter:
__
Description: __
Cloning Method: __
Quantity: 100 Reactions
Reporter Gene: __
For Use With (Application): __
Type: Assay Kit
Delivery Type: __
Content And Storage: Sufficient For: 100 luciferase assays in microplate wells• Gaussia Glow Assay Buffer, 5 mL (store at 4°C)• 100X Coelenterazine, 50 μL (store at -80°C)• Luciferase Cell Lysis Buffer (2X), 6 mL (store at room temperature)Kit may be stored at -80°C, or individually at indicated temperatures.
Selection Agent (Eukaryotic): __
Promoter: EF-1α
Description:
__
Cloning Method:
__
Quantity:
100 Reactions
Reporter Gene:
__
For Use With (Application):
__
Type:
Assay Kit
Delivery Type:
__
Content And Storage:
Sufficient For: 100 luciferase assays in microplate wells• Gaussia Glow Assay Buffer, 5 mL (store at 4°C)• 100X Coelenterazine, 50 μL (store at -80°C)• Luciferase Cell Lysis Buffer (2X), 6 mL (store at room temperature)Kit may be stored at -80°C, or individually at indicated temperatures.
Selection Agent (Eukaryotic):
__
Promoter:
EF-1α