PI16156
Thermo Scientific™ pCMV-Red Firefly Luc Vector for Luciferase Assays
Manufacturer: Thermo Scientific™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI16156-Each-of-1 | In Stock | ₹ 13,590.30 |
PI16156 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 13,590.30
GST (18%): ₹ 2,446.254
Total Price: ₹ 16,036.554
Description
pCMV-Red Firefly Luc Vector
Cloning Method
Restriction Enzyme/MCS
Quantity
10 μg
Reporter Gene
Red Firefly Luciferase
Product Line
Pierce™
Promoter
CMV
Type
Luc Vector
Delivery Type
Transfection
Content And Storage
Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C.
Selection Agent (Eukaryotic)
Puromycin
For Use With (Application)
Reporter Assays
Related Products
Description
- The Thermo Scientific™ pMCS-Red Firefly Luc Vector is a multiple cloning site plasmid designed to accept a promoter sequence for study of gene regulation using the intracellular red firefly luciferase reporter
- The Red Firefly Luc Vectors contain a mutant form of the firefly luciferase gene that has a red-shifted emission spectrum
- The pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential
- The pCMV and pTK vectors have the luciferase gene under the CMV (Cytomegalovirus) promoter and Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter, respectively
- These constitutive expression vectors can be used as normalization controls to account for experimental variation in combination with other reporters
- Highlights: Intracellular Red Firefly Luciferase gene, optimized for high expression in mammalian systems Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively High-copy pUC bacterial DNA replication origin Two control vectors available with strong (CMV) or weak (TK) constitutive promoters for co-transfection and normalization
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Description: pCMV-Red Firefly Luc Vector
Cloning Method: Restriction Enzyme/MCS
Quantity: 10 μg
Reporter Gene: Red Firefly Luciferase
Product Line: Pierce™
Promoter: CMV
Type: Luc Vector
Delivery Type: Transfection
Content And Storage: Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C.
Selection Agent (Eukaryotic): Puromycin
For Use With (Application): Reporter Assays
Description:
pCMV-Red Firefly Luc Vector
Cloning Method:
Restriction Enzyme/MCS
Quantity:
10 μg
Reporter Gene:
Red Firefly Luciferase
Product Line:
Pierce™
Promoter:
CMV
Type:
Luc Vector
Delivery Type:
Transfection
Content And Storage:
Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C.
Selection Agent (Eukaryotic):
Puromycin
For Use With (Application):
Reporter Assays
Description: pTK-Red Firefly Luc Vector
Cloning Method: Restriction Enzyme ⁄ MCS
Quantity: 10 μg
Reporter Gene: Red Firefly Luciferase
Product Line: __
Promoter: TK
Type: Luc Vector
Delivery Type: Transfection
Content And Storage: Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C.
Selection Agent (Eukaryotic): Puromycin
For Use With (Application): Reporter Assays
Description:
pTK-Red Firefly Luc Vector
Cloning Method:
Restriction Enzyme ⁄ MCS
Quantity:
10 μg
Reporter Gene:
Red Firefly Luciferase
Product Line:
__
Promoter:
TK
Type:
Luc Vector
Delivery Type:
Transfection
Content And Storage:
Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C.
Selection Agent (Eukaryotic):
Puromycin
For Use With (Application):
Reporter Assays
Description: __
Cloning Method: __
Quantity: 100 Reaction Kit
Reporter Gene: __
Product Line: Pierce™
Promoter: __
Type: Assay Kit
Delivery Type: __
Content And Storage: Sufficient For: 100 luciferase assays in microplate wells• Gaussia Flash Assay Buffer, 5 mL (store at 4°C)• 100X Coelenterazine, 50 μL (store at -80°C)• Luciferase Cell Lysis Buffer (2X), 6 mL (store at room temperature)Kit may be stored at -80°C, or individually at indicated temperatures.
Selection Agent (Eukaryotic): __
For Use With (Application): __
Description:
__
Cloning Method:
__
Quantity:
100 Reaction Kit
Reporter Gene:
__
Product Line:
Pierce™
Promoter:
__
Type:
Assay Kit
Delivery Type:
__
Content And Storage:
Sufficient For: 100 luciferase assays in microplate wells• Gaussia Flash Assay Buffer, 5 mL (store at 4°C)• 100X Coelenterazine, 50 μL (store at -80°C)• Luciferase Cell Lysis Buffer (2X), 6 mL (store at room temperature)Kit may be stored at -80°C, or individually at indicated temperatures.
Selection Agent (Eukaryotic):
__
For Use With (Application):
__
Description: __
Cloning Method: __
Quantity: 1000 Reaction Kit
Reporter Gene: __
Product Line: Pierce™
Promoter: __
Type: Assay Kit
Delivery Type: __
Content And Storage: Sufficient For: 1000 luciferase assays in microplate wells• Gaussia Flash Assay Buffer, 50 mL (store at 4°C)• 100X Coelenterazine, 0.5 mL (store at -80°C)• Luciferase Cell Lysis Buffer (2X), 60 mL (store at room temperature)Kit may be stored at -80°C, or individually at indicated temperatures.
Selection Agent (Eukaryotic): __
For Use With (Application): __
Description:
__
Cloning Method:
__
Quantity:
1000 Reaction Kit
Reporter Gene:
__
Product Line:
Pierce™
Promoter:
__
Type:
Assay Kit
Delivery Type:
__
Content And Storage:
Sufficient For: 1000 luciferase assays in microplate wells• Gaussia Flash Assay Buffer, 50 mL (store at 4°C)• 100X Coelenterazine, 0.5 mL (store at -80°C)• Luciferase Cell Lysis Buffer (2X), 60 mL (store at room temperature)Kit may be stored at -80°C, or individually at indicated temperatures.
Selection Agent (Eukaryotic):
__
For Use With (Application):
__