Thermo Scientific™ CYP2J2 BACULOSOMES™ Plus Reagent, rHuman

Description

• Cytochrome P450 BACULOSOMES™ Plus Reagents are microsomes prepared from insect cells infected with recombinant baculovirus containing a human CYP450 isozyme, as well as human cytochrome P450 reductase
• For this particular isozyme (CYP2J2), human cytochrome b5 is also included
• Single human P450 isozyme for detailed drug metabolism studies High specific activity increases dynamic range and sensitivity No background metabolism for clear results Single Overexpressed Human CYP450 Isozyme CYP450 BACULOSOMES™ Plus Reagents offer a distinct advantage over Human Liver Microsomes (HLMs) in that only one CYP450 isozyme is expressed, thereby preventing metabolism by other CYP450s or other classes of drug metabolizing enzymes
• High Activity Results in Increased Sensitivity Compound rankings based on metabolism and inhibition profiles observed with CYP450 BACULOSOMES™ Plus Reagents are very similar to those seen with HLMs for most compounds tested
• However, CYP450 BACULOSOMES™ Plus Reagents activity and metabolic rates with most substrates is significantly higher than those seen with HLMs
• This results in a broad dynamic range and high sensitivity in assays utilizing CYP450 BACULOSOMES™ Plus Reagents
• The high level of activity and reproducibility of the enzyme component observed in the reaction with most probe substrates makes CYP450 BACULOSOMES™ Plus Reagents well suited to high-throughput screening formats
• High Signal to Noise Ratio No background metabolism by endogenous insect CYP450s has been detected with any of the Vivid™ probe substrates tested so far
• Applications Isozyme identification DMPK analysis Isozyme-specific metabolism Inhibition screening