Thermo Scientific™ 1-Step Human High-Yield IVT Kit

Description

• The Thermo Scientific™ 1-Step Human High-Yield In Vitro Translation (IVT) Kits with continuous-feed device enables the expression of functional proteins with 10 to 100 times greater yield per mL than other eukaryotic IVT translation systems
• The 1-Step High-Yield IVT System uses modified HeLa cell extracts to take advantage of the robust human transcription and translation machinery and generate functional full-length proteins
• In this system, protein expression is performed in a proprietary mini dialysis device that allows a continuous supply of nucleotides, amino acids and energy generating substrates into the reaction while removing inhibitors of proteins synthesis
• This continuous-exchange cell-free (CECF) system enables protein expression for up to 24 hours and produces protein yields ranging from 250 to 750μg/mL
• The complete kits come with all components required for proper transcription and translation of recombinant protein, including an optimized expression vector
• Highlights: High expression – up to 750μg/mL of expressed protein Reproducibility – low variability between experiments Fast – express high levels of protein with an overnight incubation Functional – obtain more active protein than with bacterial protein expression systems 1-Step Protein Expression: Streamlined reaction – requires only five components Easy-to-use – transcription and translation is performed in one reaction step Adapt for labeling – amenable to incorporation of heavy or unnatural amino acids Includes: Complete kits contain cell lysate, reaction mix, accessory proteins, 5X dialysis buffer, control DNA, cloning vector, mini dialysis devices and centrifuge tubes Requires: 30°C incubator or water bath Recommended for: Production of recombinant proteins for structural analysis; Generate active proteins for protein interaction studies; Perform rapid expression and characterization of mutant proteins; Small-scale production of virual particles; Incorporation of unnatural amino acids or toxic protein production