Thermo Scientific™ 1-Step Human Coupled IVT Kit - DNA

Description

• The Thermo Scientific™ 1-Step Human In Vitro Protein Expression Kits enable the translation and post-transcriptional modification of full-length proteins from mRNA or plasmid templates with yields of up to 100μg/mL per reaction
• The Human IVT Kits are unique HeLa cell lysate-based protein expression systems for in vitro translation or coupled transcription/translation reactions
• Protein expression is performed in a single 90-minute reaction that can be extended for up to 6 hours with continued protein production up to 100μg/mL when combined with the optimized pT7CFE1 Expression Vector
• The Human IVT Kits can express functional enzymes, phosphoproteins, glycoproteins and membrane proteins for immediate use in studying protein interactions, performing rapid mutational analysis and measuring activity
• Highlights: Functional – uses the human translational machinery to express active proteins Convenient – perform transcription and translation in a single step High performance – greater yields compared to rabbit reticulocyte in vitro translation Reliable – express proteins that fail in rabbit reticulocyte systems Better Than Traditional Methods: HeLa cell-free extracts are capable of expressing proteins with post-translational modifications Accurate translation delivers full-length protein compatible with downstream applications Protein translation is optimized with EMCV IRES element and other mRNA stabilizing elements Includes: Complete kits contain HeLa cell lysate, accessory proteins, reaction mix, nuclease-free water, expression vector and a GFP-positive control vector Requires: 30°C incubator or water bath Recommended for: Express proteins to measure enzyme activity; Perform mutational analysis; Express protein for use in gel mobility shift assays; Perform co-immunoprecipitation; Express cytotoxic proteins; Perform unnatural amino acid labeling