4926794

Invitrogen™ Ni-NTA Agarose

Nickel-charged affinity resin that can be used to purify recombinant proteins containing a polyhistidine (6xHis) sequence

Manufacturer: Fischer Scientific

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Content And Storage

The Ni-NTA resin is pre-charged and able to bind up to 50 mg of recombinant protein per 1 ml of resin. It is provided as a 50% slurry in 30% ethanol. The resin will appear blue in color when charged with Ni2+. Store at +4°C. Ni-NTA resin is guaranteed stable for 6 months when properly stored.

Type

Agarose

Column Type

Affinity Column

Stationary Phase

Ni-NTA

Form

Suspension

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Description

  • Ni-NTA Agarose is a nickel-charged affinity resin that can be used to purify recombinant proteins containing a polyhistidine (6xHis) sequence Proteins bound to the resin may be eluted with either low pH buffer or by competition with imidazole or histidine One-step purification can be performed under both native and denaturing conditions Ni-NTA uses the chelating ligand nitrilotriacetic acid (NTA) coupled to a cross-linked 6% agarose resin that is suitable for use in batch and gravity flow applications Guaranteed stable for 6 months when properly stored at 4°C

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Content And Storage:
The Ni-NTA resin is pre-charged and able to bind up to 50 mg of recombinant protein per 1 ml of resin. It is provided as a 50% slurry in 30% ethanol. The resin will appear blue in color when charged with Ni2+. Store at +4°C. Ni-NTA resin is guaranteed stable for 6 months when properly stored.

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Agarose

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Affinity Column

Stationary Phase:
Ni-NTA

Form:
Suspension

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