7182886

Invitrogen™ ProBond™ Nickel-Chelating Resin

A nickel-charged affinity resin used to purify recombinant proteins containing a polyhistidine (6xHis) sequence.

Manufacturer: Fischer Scientific

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Content And Storage

The ProBond™ resin is pre-charged and able to bind 1-5 mg of recombinant protein per 1 ml of resin. It is provided as a 50% slurry in 20% ethanol. The resin will appear blue in color when charged with Ni2+. Store at +4°C. ProBond™ resin is guaranteed stable for 6 months when properly stored.

Product Line

ProBond™

Form

Suspension

Stationary Phase

Nickel-Chelating

Type

Resin

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Description

  • ProBond™ Nickel-Chelating Resin is a nickel-charged affinity resin used to purify recombinant proteins containing a polyhistidine (6xHis) sequence Proteins bound to the resin may be eluted with either low pH buffer or by competition with imidazole or histidine One-step purification can be performed under both native and denaturing conditions ProBond™ Resin uses the chelating ligand iminodiacetic acid (IDA) coupled to a highly cross-linked 6% agarose resin Suitable for use in FPLC, batch, and gravity flow applications Guaranteed stable for 6 months when properly stored at 4°C

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Content And Storage:
The ProBond™ resin is pre-charged and able to bind 1-5 mg of recombinant protein per 1 ml of resin. It is provided as a 50% slurry in 20% ethanol. The resin will appear blue in color when charged with Ni2+. Store at +4°C. ProBond™ resin is guaranteed stable for 6 months when properly stored.

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ProBond™

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Suspension

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