Thermo Scientific™ CaptureSelect™ KappaXP MiniChrom™ Pre-packed columns

Description

• CaptureSelect KappaXP MiniChrom™ column is designed for bench-scale resin screening, process development, and sample preparation
• This pre-packed column is ready to connect and has the following features: Volume of 5 mL (0.8 x 10 cm) Direct connection to standard chromatography systems Packed with CaptureSelect KappaXP Affinity Matrix, offering high dynamic binding capacity and high resolution over a wide range of flow rates The 10-cm bed height of the 5-mL column allows initial process development on a bench scale
• CaptureSelect KappaXP matrix recognizes the kappa light chain of human antibodies, enabling purification of all Kappa containing Ig's, Fab, and Fab2 fragments directly from complex sources and feed streams
• Features of this affinity matrix include: Purification of Kappa monoclonal antibodies, Fab, and Fab2 fragments in a single step with high purity and yield Mild elution for Fab fragments and antibodies Human-specific, no binding to bovine antibodies Excellent scalability Excellent pH stability up to 100 mM NaOH (limited exposure) Non-animal derived Free of animal components CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology
• The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free)
• Main characteristics Matrix: agarose-based, epoxide-activated Average particle size: 65 ± 10 μm Ligand: CaptureSelect KappaXP affinity ligand Ligand coupling method: epoxide coupling Fab binding capacity: 20–30 g IgG Kappa-Fab/liter resin depending on flow rate, column height, and residence time IgG binding capacity: 35–45 g IgG/liter resin depending on flow rate, column height Elution conditions: 20 mM citric or acetic acid, pH 3–4; 100 mM Tris, 1.5 M MgCl 2 , pH 6 Column dimensions: 5 mL (0.8 x 10 cm) Flow characteristics: 75–150 cm/h (up to 2 bar) Formulation buffer: 20% (v/v) ethanol