Gibco™ ExpiCHO-S™ Cells

Description

• The ExpiCHO Expression System Kit combines the power of rapid, ultra–high-yield transient protein production in suspension culture with the benefit of expression in Chinese hamster ovary (CHO) cells
• It is based on high-density culture of ExpiCHO-S cells in ExpiCHO Expression Medium
• Transient expression is powered by ExpiFectamine CHO transfection reagent, designed specifically for high-density CHO suspension culture, in combination with a specialized transfection enhancer and culture feed
• All components work together synergistically with expression yields of 1–3 g per L of transfected culture demonstrated for some antibody and non-antibody proteins
• The ExpiCHO Expression System enables: Milligram to multigram yields of recombinant protein per liter of culture in 5–14 days post-transfection Robust and reproducible transfection results , for greater confidence in results and ability to use CHO cells from research to discovery Transfection efficiency of > 90% for high-density suspension CHO cell culture, with matching transfection enhancer and culture feed to boost transfection performance and protein expression Use of one-third to one-half less plasmid DNA than other transfection reagents Flexible, reliable culture and transfection protocols with multiple options that readily fit into current transient expression workflows Scalable expression for culture formats from  <1 mL in deep well plates up to 5 L when using Nalgene angled-bottom shake flasks The components included in the ExpiCHO Expression System Kit are: 2 vials of frozen ExpiCHO-S cells, 1 L of ExpiCHO Expression Medium, 1 ExpiFectamine CHO Transfection Kit sufficient to transfect 1 L of culture, OptiPRO SFM, and an antibody-expressing positive control vector
• ExpiCHO-S cells, ExpiCHO Expression Medium, ExpiFectamine CHO Transfection Kit, and OptiPRO SFM may also be purchased separately
• All components of the system are animal origin–free.