Thermo Scientific™ Pierce™ c-Myc Tag IP/Co-IP Kit

Description

• The c-Myc peptide (EQKLISEEDL) has become a popular fusion tag for mammalian recombinant protein expression, and this kit facilitates immunoprecipitation (IP) or co-immunoprecipitation (co-IP) experiments involving c-Myc-tagged proteins
• The kit includes crosslinked beaded agarose to which a highly specific anti-c-Myc antibody is covalently immobilized
• Upon incubation with a sample containing the tagged fusion protein, interaction complexes involving the c-Myc-tagged bait protein are captured on the agarose beads
• After simple washing steps, the specific protein interaction complex is easily eluted from the resin in the supplied elution buffer or SDS-PAGE sample loading buffer for subsequent analysis
• The kit includes the prepared agarose affinity resin, buffers, microcentrifuge spin columns, a positive control and easy-to-follow instructions
• Highlights: Specific – the immobilized anti-c-Myc monoclonal antibody binds the c-Myc epitope tag with high specificity, providing high yield immunoprecipitation products and clean Western blot detection High capacity – excellent results with as little as 1μg of anti-c-Myc antibody in IP mode with the positive control lysate Rigorous – kit includes a positive control lysate that contains over-expressed GST-c-Myc to validate the reagents and specific protocol, and the instructions provide tips for planning other controls Robust – the affinity system is compatible with IP or Co-IP from various cell lysates and physiological (non-denaturing) buffer systems; choose kits with or without mammalian cell lysis buffer Convenient and easy – complete kit includes all necessary reagents, convenient spin columns, and easy-to-follow instructions