Thermo Scientific™ Pierce™ c-Myc-Tag Magnetic IP/Co-IP Kit

Description

• The Thermo Scientific Pierce Magnetic c-Myc-Tag IP/Co-IP Kit contains specific immunoaffinity magnetic beads and reagents to perform immunoprecipitation assays of c-Myc fusion proteins or co-IP experiments using c-Myc-tagged bait proteins
• Unlike traditional IP procedures based on capture with Protein A/G beads, this kit uses magnetic beads containing pre-immobilized anti-c-Myc antibody
• These Pierce Anti-c-Myc Magnetic Beads ensure specific binding of c-Myc tagged protein complexes from biological samples
• Because the antibody is covalently attached to the beads, IP-targets are easily eluted and recovered without antibody contamination
• The complete IP kit includes the magnetic beads, lysis/wash buffer, low-pH elution buffer, neutralization buffer, c-Myc-tag positive control lysate, and non-reducing sample buffer for SDS-PAGE
• Protocols are provided for both manual and automated magnetic separation workflows
• Sufficient components are provided to perform 40 IP or co-IP assays
• Highlights: Specific magnetic beads - covalently immobilized high-quality anti-c-Myc monoclonal antibody enables high yields of immunoprecipitation products Low non-specific binding - stable, pre-blocked beads and specific antibody minimize off-target binding Trouble-free elution - low-pH elution buffer ensures recovery of c-Myc-tagged protein interaction complexes without antibody leaching contamination Convenient and fast - complete kit and easy-to-follow instructions provide optimized protocols to perform IP or Co-IP experiment in approximately 1 hour Versatile - magnetic beads are compatible with manual and automated magnetic separation workflows (e.g., Thermo Scientific KingFisher Instruments) Product Details: The c-Myc peptide (EQKLISEEDL) derived from the C-terminus region of human c-Myc protein is one of several fusion protein tags used for recombinant protein expression
• The Pierce c-Myc Magnetic IP/Co-IP Kit uses a specific, high-affinity immobilized antibody (clone 9E10) for rapid immunoprecipitation of c-Myc tagged fusion proteins from bacterial and mammalian cell lysates, as well as from lysates prepared with the Pierce Human in vitro Translation Kits
• The beads are incubated with a cell lysate containing c-Myc tagged protein, the fusion protein is captured, and the beads are subsequently washed and then eluted using low-pH elution buffer or non-reducing sample buffer
• The protocol and buffers have been optimized for both IP and co-IP reactions, enriching for specific protein interaction complexes in the eluted samples
• Anti-c-Myc antibody can be used to detect c-Myc tagged protein by Western blot analysis
• For information about the binding capacity and other properties of the magnetic beads used in this kit, see Pierce Anti-c-Myc Magnetic Beads (Part No
• 88842).