Thermo Scientific™ pT7CFE1-CHis Vector for Mammalian Cell-Free Protein Expression

Description

• Thermo Scientific™ T7 Cell-Free Expression Vectors (pT7CFE1) are cloning plasmids optimized to use with the Thermo Scientific 1-Step Human In Vitro Protein Expression System for in vitro translation (IVT) of tagged fusion proteins
• The pT7CFE1 Expression Vectors contain the Encephalomyocarditis virus (EMCV) internal ribosome entry site (IRES) element that is critical for high levels of cap-independent protein expression in the Human In Vitro Translation System
• Each vector features a highly-compatible multiple cloning site (MCS) to facilitate easy insertion of protein coding sequences into and between vectors
• The pT7CFE1 Vector is available with single or tandem affinity tags at the N- or C- terminus to facilitate protein purification and detection
• The pT7CFE Vectors are suitable for insertion of cloned genes, cDNAs, ORFs or PCR products for in vitro transcription and translation
• Custom cloning services are also available
• Highlights: Optimized performance – designed to provide the highest yield in the human in vitro translation system Many options – multiple tag and tag-location options available Modular MCS – multiple cloning site is maintained across vector family to facilitate subcloning Cleavable tags – HRV 3C cleavage site available on select vectors pT7CFE1 Plasmid Features: EMCV IRES at the 5-prime UTR promotes high-level translation of mRNAs MCS accommodates gene insertion via ten different restriction sites: Msc1, Nde1, BamH1, EcoR1, EcoRV, Pac1, Pst1, Sac1, Sal1, Not1 and Xho1 Poly A sequence in the 3-prime region promotes mRNA stabilization and protection from nucleases T7 terminator ensures synthesis of accurate size mRNA transcripts Plasmid linearization may be accomplished with restriction sites between Poly A sequence and the T7 terminator region