Thermo Scientific™ Pierce™ Protein A Agarose

Description

• Perform a variety of antibody affinity purification methods with Thermo Scientific™ Pierce Protein A Agarose, a standard-capacity Protein A beaded agarose resin
• Pierce Protein A Agarose consists of purified native Protein A that has been covalently immobilized onto high-quality crosslinked 6% beaded agarose
• The agarose beads have physical and chemical properties that enable them to be used in a variety of batch- or column-type affinity purification procedure
• Several package formats are offered: 5mL and 25mL bottles of resin slurry, 1mL pre-packed columns and a complete IgG purification kit
• Protein A is a bacteria-derived protein that binds with high affinity and specificity to the Fc portion of antibodies, especially those of the IgG class
• Compared to its alternative (Protein G), Protein A provides the highest binding capacity for subclasses of IgG from rabbits, pigs, dogs and cats
• Protein A also has higher binding capacity for human IgG, except for IgG3, which it binds weakly
• Protein A also binds weakly to mouse IgG1 and is not recommended for most applications with that antibody isotype
• Highlights: Native Protein A – immobilized Protein A is ideal for polyclonal IgG purification from human, rabbit, pig, dog or cat serum Agarose resin – support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods Inert and stable – superior manufacturing method immobilizes Protein A by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield Standard capacity – Pierce Protein A Agarose has a normal load of immobilized Protein A, providing a binding capacity of 12 to 19mg human IgG/mL resin (approx
• 6 to 8mg mouse IgG/mL resin) Properties of crosslinked 6% beaded agarose (CL-6B): Support pH Stability: 2 to 14 (short term); 3 to 13 (long term) Average Particle Size: 45 to 165 microns Exclusion Limit: 10,000 to 4,000,000 daltons Maximum Volumetric Flow Rate: approx
• 1mL/minute (for 1cm diameter column) Maximum Linear Velocity: 30cm per hour Maximum Pressure: less than 25psi (1.5 bar), defined as the maximum pressure drop across a column that the resin can withstand (Note: The indicated gauge pressure of a liquid chromatography apparatus may be measuring the total system pressure rather than the pressure drop across the column.) Available Formats: Resin Slurries: crosslinked 6% beaded agarose; 5mL and 25mL bottles Spin Columns: 1mL columns Purification Kit: complete kit containing five 1mL columns Bulk and Custom: let us know your needs for bulk Pierce Protein A Agarose Includes: Kit contains Protein A Column (1mL each), Protein A IgG Binding Buffer, IgG Elution Buffer and desalting columns