Thermo Scientific™ Pierce™ Recombinant Protein A Agarose

Description

• Thermo Scientific™ Pierce Recombinant Protein A Agarose is a beaded agarose resin to which a genetically engineered form of Protein A has been immobilized for use in IgG purification applications where the native ligand has undesirable effects
• The Pierce Recombinant Protein A Agarose uses a genetically engineered form of Protein A that is produced in E
• coli and then immobilized on crosslinked 6% beaded agarose (CL-6B)
• Although some nonessential regions have been removed, recombinant is similar to native Protein A in overall size and IgG-binding domains
• The protein has four high affinity binding sites capable of specifically binding to the Fc region of immunoglobulin molecules from several species
• Some researchers prefer to use an affinity resin based on the recombinant form of Protein A in particular applications where practical experience indicates that the different production source ( E
• coli vs
• S
• aureus ) provides less nonspecific binding or lower levels of endotoxin contamination
• Otherwise, native Protein A resins generally provide higher capacity antibody purification
• Highlights: Immobilized Protein A – specifically binds IgG from several mammalian species, especially most subtypes of human and rabbit IgG Standard capacity – loading of immobilized Protein A provides a binding capacity of 15 to 17mg human IgG per milliliter of resin Agarose resin – crosslinked 6% beaded agarose for small column or batch affinity purification methods Properties of crosslinked 6% beaded agarose (CL-6B): Support pH Stability: 2 to 14 (short term); 3 to 13 (long term) Average Particle Size: 45 to 165 microns Exclusion Limit: 10,000 to 4,000,000 daltons Maximum Volumetric Flow Rate: approx
• 1mL/minute (for 1cm diameter column) Maximum Linear Velocity: 30cm per hour Maximum Pressure: less than 25psi (1.5 bar), defined as the maximum pressure drop across a column that the resin can withstand (Note: The indicated gauge pressure of a liquid chromatography apparatus may be measuring the total system pressure rather than the pressure drop across the column.)