Thermo Scientific™ Pierce™ Protein G Agarose

Description

• Thermo Scientific™ Pierce Protein G Agarose is a versatile, high-performance affinity resin for antibody purification that is available as bottled agarose beads, pre-packed spin columns, 96-well filter plates and complete IgG purification kits
• Pierce Protein G Agarose is effective for affinity purification of IgG from serum and other fluids of many mammalian species
• Protein G is especially well suited for use with mouse antibodies (including IgG1) in addition to most IgG isotypes from human, goat and sheep samples
• Pierce Protein G Agarose consists of recombinant Protein G that has been covalently immobilized onto high-quality crosslinked 6% beaded agarose (CL-6B)
• This premiere variety of the affinity resin provides the most versatile combination of chromatographic features for high yield and high purity purification of whole IgG from mammalian serum samples
• The agarose beads have physical and chemical properties suitable for many affinity purification systems
• Accordingly, Pierce Protein G Agarose is offered in several convenient package formats, including bottled resin slurries, three sizes of spin columns, complete purification kits, two sizes of FPLC-ready chromatography cartridges, and 96-well filter plates
• Highlights: Protein G – immobilized Protein G is ideal for polyclonal IgG purification from mouse, human, cow, goat and sheep serum, including human IgG3 and mouse IgG1 isotypes Agarose resin – support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods Inert and stable – superior manufacturing method immobilizes Protein G by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield Standard capacity – Pierce Protein G Agarose has a normal load of immobilized Protein G, providing a binding capacity of 11 to 15mg human IgG/mL resin Multiple formats – choose from bottled resin slurries, centrifuge-ready columns, complete purification kits, two sizes of FPLC-ready chromatography cartridges, and 96-well filter plates Properties of crosslinked 6% beaded agarose (CL-6B): Support pH Stability: 2 to 14 (short term); 3 to 13 (long term) Average Particle Size: 45 to 165 microns Exclusion Limit: 10,000 to 4,000,000 daltons Maximum Volumetric Flow Rate: approx
• 1mL/minute (for 1cm diameter column) Maximum Linear Velocity: 30cm per hour Maximum Pressure: less than 25psi (1.5 bar), defined as the maximum pressure drop across a column that the resin can withstand (Note: The indicated gauge pressure of a liquid chromatography apparatus may be measuring the total system pressure rather than the pressure drop across the column.) Available Formats: Resin Slurries: 2mL, 10mL, 25mL bottles Spin Columns: 0.2mL (microcentrifuge), 1mL, 5mL columns Purification Kits: complete kits in all three column sizes Chromatography Cartridges: compatible with automated liquid chromatography systems and with syringe processing 96-well Spin Plates: pre-dispensed 96-well filter plates, compatible with centrifugation and vacuum manifold systems for manual or automated purification
• The plates enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput separations
• Bulk and Custom: let us know your needs for bulk Pierce Protein G Agarose Includes: Purification kits contain Protein G Spin Columns, binding buffer, elution buffer and neutralization buffer