Thermo Scientific™ Pierce™ Protein L Agarose

Description

• Thermo Scientific™ Pierce Protein L Agarose is a versatile, high-performance affinity resin for antibody purification that is available as bottled agarose beads, pre-packed spin columns, 96-well filter plates and complete IgG purification kits
• Pierce Protein L Agarose is a versatile affinity resin for purification of mammalian IgG isotypes that contain specific kappa light chains
• Protein L is especially suited for isolating mouse IgG VkI and human IgG VkI, VkIII and VkIV monoclonal antibodies
• Pierce Protein L Agarose consists of recombinant Protein L that has been covalently immobilized onto high-quality crosslinked 6% beaded agarose (CL-6B)
• This premiere variety of the resin provides the most versatile combination of chromatographic features for high yield and high purity purification of target immunoglobulins
• The agarose beads have physical and chemical properties suitable for many affinity purification systems
• Accordingly, Pierce Protein L Agarose is offered in several convenient package formats, including bottled resin slurries, three sizes of spin columns, complete purification kits, two sizes of FPLC-ready chromatography cartridges, and 96-well filter plates
• Highlights: Protein L – immobilized Protein L is ideal for selective purification of human and mouse antibodies that have kappa light chains Agarose resin – support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods Inert and stable – superior manufacturing method immobilizes Protein L by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield Standard capacity – Pierce Protein L Agarose has a normal load of immobilized Protein L, providing a binding capacity of 5 to 10mg human IgG/mL resin Multiple formats – choose from bottled resin slurries, centrifuge-ready columns, complete purification kits and two sizes of FPLC-ready chromatography cartridges Immunoglobulin-binding properties of Protein L: Binds to kappa light chains on antibodies from a wide range of species without interfering with antigen-binding sites; does not bind lambda light chains and some kappa light chains) Binds to all classes of immunoglobulin (e.g., IgG, IgM, IgA, IgE and IgD) Binds single-chain variable fragments (ScFv) Does not bind bovine, goat or sheep immunoglobulins Properties of crosslinked 6% beaded agarose (CL-6B): Support pH Stability: 2 to 14 (short term); 3 to 13 (long term) Average Particle Size: 45 to 165 microns Exclusion Limit: 10,000 to 4,000,000 daltons Maximum Volumetric Flow Rate: approx
• 1mL/minute (for 1cm diameter column) Maximum Linear Velocity: 30cm per hour Maximum Pressure: less than 25psi (1.5 bar), defined as the maximum pressure drop across a column that the resin can withstand (Note: The indicated gauge pressure of a liquid chromatography apparatus may be measuring the total system pressure rather than the pressure drop across the column.) Available Formats: Resin Slurries: 2mL and 10mL bottles Spin Columns: 0.2mL (microcentrifuge), 1mL, 5mL columns Purification Kits: complete kits in all three column sizes Chromatography Cartridges: compatible with automated liquid chromatography systems and with syringe processing Bulk and Custom: let us know your needs for bulk Pierce Protein L Agarose Includes: Purification kits contain Protein L Spin Columns, binding buffer, elution buffer and neutralization buffer