Anti-CD45 Antibody, clone F10-89-4

Description

• General description: CD45 is a receptor-tyrosine phosphatase required for antigen-induced signaling in T-cells and B-cells; its substrates are unknown. Interestingly, it associates with several Src-family tyrosine kinases, which are inhibited by tyrosine phosphorylation in their c-termini, suggesting a mechanism for signaling by CD45.
• Specificity: This antibody reacts with the leucocyte common antigen (LCA) complex to be found on lymphocytes, monocytes, granulocytes, thymocytes and malignant T and B cells. No reactivity has been observed with primary or metastatic carcinoma cells. Plasma cells or myeloma cells may have weak expression or be negative for this antigen. The LCA comprises of at least 5 isoforms ranging from 180-220 kDa which are produced as a result of alternative splicing of exons A, B or C. CBL 124 recognizes epitopes common to all of the isoforms. Antigen distribution: Thymocytes >95% Granulocytes >95% Monocytes >95% B cells (CD20+) >95% T cells (CD3+) >95% NK cells (CD16+) >95% Peripheral blood lymphocytes >95% FUSION PARTNER: NS1 myeloma cell line
• Immunogen: Purified T cells from human lymph nodes
• Application: Anti-CD45 Antibody, clone F10-89-4 detects level of CD45 & has been published & validated for use in FC, WB, IF, IP, IH(P).
• Quality: Routinely evaluated by Western Blot on jurkat cell lysate.Western Blot Analysis: 1:500 dilution of this lot detected CD45 on 10 μg of Jurkat lysates
• Target description: ~180-220 kDa
• Linkage: Replaces: 04-1102
• Physical form: Protein A affinity chromatography
• Storage and Stability: For use within 1 month of purchase store at 2-8°C, for long term storage aliquot antibody into small volumes and store at -20°C for up to one year from date of receipt.Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
• Analysis Note: ControlJurkat cell lysate
• Other Notes: Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.