Anti-Sodium-Iodide Symporter Antibody, C-terminal 2

Description

• General description: Sodium/iodide cotransporter (UniProt: Q92911; also known as Na(+)/I(-) cotransporter, Sodium-iodide symporter, Na(+)/I(-) symporter) is encoded by the SLC5A5 (also known as NIS) gene (Gene ID: 6528) in human. Sodium/iodide cotransporter is a multi-pass membrane protein that mediates iodide uptake in thyroid gland. It is expressed primarily in thyroid tissue, but is present in lower levels in mammary gland and ovary. It expression is significantly reduced in tumors. The presence of symporter in mammary glands leads to secretion of iodine in milk that is required for thyroid function in neonatal animals. NIS gene and protein expression is stimulated by thyroid-stimulating hormone. Both human and rat NIS contain N-glycosylation sites, however, glycosylation is not required for its full activity. Abnormalities in the expression of NIS gene lead to thyroid disease and can result in congenital hypothyroidism. Autoantibodies to NIS adversely affect iodide transport.
• Specificity: This rabbit polyclonal antibody detects Sodium-iodide symporter in human. It targets an epitope within 13 amino acids from the C-terminal end. For Western blotting application, please do not boil the sample to avoid any aggregation. When transfering protein to nitrocellulose membrane use of 0.05% (w/v) lithium dodecyl sufate is highly recommended in the transfer buffer.
• Immunogen: Epitope: C-terminus
• Application: Anti-Sodium-Iodide Symporter, C-terminal 2, Cat. No. ABC1454, is a rabbit polyclonal antibody that detects Sodium/iodide cotransporter and has been tested for use in Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
• Quality: Evaluated by Immunohistochemistry in human stomach tissue sections.Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Sodium-Iodide Symporter in human stomach tissue sections.
• Target description: 68.66 kDa calculated.
• Physical form: Affinity Purified
• Storage and Stability: Stable for 1 year at 2-8°C from date of receipt.
• Other Notes: Concentration: Please refer to lot specific datasheet.
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.