Anti-IFIT1/p56 Antibody

Description

• General description: IFIT1, also known as GARG-16, Glucocorticoid-attenuated response gene 16 protein or Interferon-induced 56 kDa protein, IFI-56K, or P56, and encoded by the gene lfit1/Garg16, Ifi56, Isg56, is an interferon-induced antiviral RNA-binding protein that specifically binds single-stranded RNA bearing a 5′-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs and inhibiting expression of viral messenger RNAs. Single-stranded PPP-RNAs, which lack 2′-O-methylation of the 5′ cap and bear a 5′-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. IFIT1 directly binds PPP-RNA in a non-sequence-specific manner. Viruses evolved several ways to evade this restriction system such as encoding their own 2′-O-methylase for their mRNAs or by stealing host cap containing the 2′-O-methylation (cap snatching mechanism). IFIT1 is a component of an interferon-dependent multiprotein complex that is at least composed of IFIT1, IFIT2 and IFIT3 and interacts with EIF3F, RPL15, TMEM173 and EEF1A1 and is important in antiviral defense, and innate immune response. IFIT1 is localized to the cytoplasm and expressed by most cells, particularly in the immune system and epithelial cells.
• Immunogen: GST-tagged recombinant protein corresponding to mouse IFIT1/p56.
• Application: Detect IFIT1 using this rabbit polyclonal antibody, Anti-IFIT1/p56 Antibody validated for use in western blotting, ICC & Flow Cytometry.
• Quality: Evaluated by Western Blotting in mouse ovary tissue lysate.Western Blotting Analysis: A 1:1,000 dilution of this antibody detected IFIT1/p56 in 10 µg of mouse ovary tissue lysate.
• Target description: ~56 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
• Physical form: Rabbit polyclonal serum containing 0.05% sodium azide.
• Storage and Stability: Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.