Anti-Actin Antibody, Alexa Fluor™ 488 Conjugate

Description

• General description: Actins are globular multi-functional proteins that serve as the basic building blocks of cytoskeletal microfilaments and are among the most conserved eukaryotic proteins. Six actin types exist, skeletal muscle alpha-actin (ACTA1), smooth muscle alpha-actin (ACTA2), cytoplasmic beta-actin (ACTB), cardiac muscle alpha-actin (ACTC1), cytoplasmic gamma-actin (ACTG1), and smooth muscle gamma-actin (ACTG2, a.k.a. ACTA3). Although actins show more than 90% overall sequence homology, isotypes do show spatial, temporal, and tissue-specific expresson patterns and only 50-60% homology is found in their 18 N-terminal residues. Cytoplasmic β and γ-actins are thought to be present in all cells, while the other four actin isoforms are typically found in specific adult muscle tissue types. Actins exist in a variety of structural states, depending on the specific ionic conditions or the interaction with ligand proteins. The oligomeric and polymeric forms that actin molecules assume are dependent on the distinct conformations they adopt. In additon to serving as the basic building blocks of cytoskeletal microfilaments, actins are involved in multiple nuclear functions and adopt specific conformations that are important for their association with nuclear actin-binding proteins.
• Specificity: Broad species reactivity expected due to 100% sequence homology, including Bovine, Horse, Non-Human Primate, Porcine, Rabbit, and Rat.
• Immunogen: Epitope: N-terminus
• Application: Flow Cytometry Analysis: 2 µL from a representative lot detected actin in one million HeLa cells.Immunofluorescence Analysis: A 1:150 dilution from a representative lot detected actin in human heart and skin muscle tissue sections.The unconjugated antibody (Cat. No. ABT1485) and Alexa Fluor™ 647-conjugated antibody (Cat. No. ABT1485-AF647) are also available for flow cytometry, immunocytochemistry, immunofluorescence, immunohistochemistry and Western blotting applications.
• Quality: Evaluated by Immunocytochemistry in HeLa cells.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected both cytoplasmic and nuclear actin immunoreactivity in HeLa cells.
• Target description: ~42 kDa observed.
• Physical form: Affinity purified.
• Storage and Stability: Stable for 1 year at 2-8°C from date of receipt.
• Other Notes: Concentration: Please refer to lot specific datasheet.
• Legal Information: ALEXA FLUOR is a trademark of Life Technologies
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.