ABT1485AF64

MilliporeSigma™ Actin, Rabbit, Alexa Fluor 647, Polyclonal,

Manufacturer: MilliporeSigma™

Select a Size

Pack Size SKU Availability Price
Each of 1 ABT1485AF64-Each-of-1 In Stock ₹ 47,563.66

ABT1485AF64 - Each of 1

₹ 47,563.66

In Stock

Quantity

1

Base Price: ₹ 47,563.66

GST (18%): ₹ 8,561.459

Total Price: ₹ 56,125.119

Antigen

Actin

Classification

Polyclonal

Conjugate

Alexa Fluor 647

Gene

ACTA1, ACTA, CFTD, NEM3, MPFD

Host Species

Rabbit

Purification Method

Affinity Purified

Regulatory Status

RUO

Primary or Secondary

Primary

Test Specificity

This Alexa Fluor™ 647 conjugate was prepared from purified rabbit polyclonal antibody (Mfr. No. ABT1485) raised against skeletal muscle alpha-actin (ACTA1) N-terminal end sequence that shares 80% sequence identity in the corresponding region with cardiac muscle alpha-actin (ACTC1), smooth muscle alpha-actin (ACTA2), and cytoplasmic gamma-actin (ACTG1). Immunogen sequence is not found in smooth muscle gamma-actin (ACTG2, a.k.a. ACTA3) and cytoplasmic beta-actin (ACTB).

Content And Storage

Stable for 1 year at 2°-8°C from date of receipt.

Applications

Immunocytochemistry, Immunofluorescence

Concentration

Please refer to lot specific datasheet.

Formulation

Purified rabbit polyclonal antibody conjugate in PBS with 1.5% BSA and 0.05% Sodium Azide.

Gene Accession No.

P68133

Immunogen

KLH-conjugated linear peptide corresponding to the N-terminal end sequence of mature skeletal muscle alpha-actin (ACTA1).

Quantity

100 μL

Research Discipline

Cell Structure

Gene ID (Entrez)

NP_001091

Target Species

Human

Form

Purified

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Description

  • Actins are globular multi-functional proteins that serve as the basic building blocks of cytoskeletal microfilaments and are among the most conserved eukaryotic proteins
  • Six actin types exist, skeletal muscle alpha-actin (ACTA1), smooth muscle alpha-actin (ACTA2), cytoplasmic beta-actin (ACTB), cardiac muscle alpha-actin (ACTC1), cytoplasmic gamma-actin (ACTG1), and smooth muscle gamma-actin (ACTG2, a.k.a
  • ACTA3)
  • Although actins show more than 90% overall sequence homology, isotypes do show spatial, temporal, and tissue-specific expresson patterns and only 50-60% homology is found in their 18 N-terminal residues
  • Cytoplasmic β and γ-actins are thought to be present in all cells, while the other four actin isoforms are typically found in specific adult muscle tissue types
  • Actins exist in a variety of structural states, depending on the specific ionic conditions or the interaction with ligand proteins
  • The oligomeric and polymeric forms that actin molecules assume are dependent on the distinct conformations they adopt
  • In additon to serving as the basic building blocks of cytoskeletal microfilaments, actins are involved in multiple nuclear functions and adopt specific conformations that are important for their association with nuclear actin-binding proteins.

Compare Similar Items

Show Difference

Img

MilliporeSigma™

ABT1485AF64

--


Antigen:
Actin

Classification:
Polyclonal

Conjugate:
Alexa Fluor 647

Gene:
ACTA1, ACTA, CFTD, NEM3, MPFD

Host Species:
Rabbit

Purification Method:
Affinity Purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
This Alexa Fluor™ 647 conjugate was prepared from purified rabbit polyclonal antibody (Mfr. No. ABT1485) raised against skeletal muscle alpha-actin (ACTA1) N-terminal end sequence that shares 80% sequence identity in the corresponding region with cardiac muscle alpha-actin (ACTC1), smooth muscle alpha-actin (ACTA2), and cytoplasmic gamma-actin (ACTG1). Immunogen sequence is not found in smooth muscle gamma-actin (ACTG2, a.k.a. ACTA3) and cytoplasmic beta-actin (ACTB).

Content And Storage:
Stable for 1 year at 2°-8°C from date of receipt.

Applications:
Immunocytochemistry, Immunofluorescence

Concentration:
Please refer to lot specific datasheet.

Formulation:
Purified rabbit polyclonal antibody conjugate in PBS with 1.5% BSA and 0.05% Sodium Azide.

Gene Accession No.:
P68133

Immunogen:
KLH-conjugated linear peptide corresponding to the N-terminal end sequence of mature skeletal muscle alpha-actin (ACTA1).

Quantity:
100 μL

Research Discipline:
Cell Structure

Gene ID (Entrez):
NP_001091

Target Species:
Human

Form:
Purified

Img

MilliporeSigma™

ABT1485MI

--


Antigen:
Actin

Classification:
Polyclonal

Conjugate:
Unconjugated

Gene:
ACTA1, ACTA, CFTD, NEM3, MPFD

Host Species:
Rabbit

Purification Method:
Affinity Purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
This rabbit polyclonal antibody was raised against skeletal muscle alpha-actin (ACTA1) N-terminal end sequence that shares 80% sequence identity in the corresponding region with cardiac muscle alpha-actin (ACTC1), smooth muscle alpha-actin (ACTA2), and cytoplasmic gamma-actin (ACTG1). Immunogen sequence is not found in smooth muscle gamma-actin (ACTG2, a.k.a. ACTA3) and cytoplasmic beta-actin (ACTB).

Content And Storage:
Stable for 1 year at 2°-8°C from date of receipt.

Applications:
Immunohistochemistry (Paraffin), Western Blot

Concentration:
Please refer to lot specific datasheet.

Formulation:
Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.

Gene Accession No.:
P68133

Immunogen:
KLH-conjugated linear peptide corresponding to the N-terminal end sequence of mature skeletal muscle alpha-actin (ACTA1).

Quantity:
100 μL

Research Discipline:
Cell Structure

Gene ID (Entrez):
NP_001091

Target Species:
Human

Form:
__

Img

MilliporeSigma™

ABT1486

--


Antigen:
CD31/PECAM-1

Classification:
Polyclonal

Conjugate:
__

Gene:
__

Host Species:
Rabbit

Purification Method:
Affinity Purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
__

Content And Storage:
Stable for one year at 2°C to 8°C from date of receipt.

Applications:
Flow Cytometry, Western Blot

Concentration:
__

Formulation:
Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.

Gene Accession No.:
P16284

Immunogen:
KLH-conjugated linear peptide corresponding to a cytoplasmic domain seqence of human CD31/PECAM-1.

Quantity:
100 μg

Research Discipline:
Cell Structure

Gene ID (Entrez):
NP_000433

Target Species:
Human

Form:
__

Img

MilliporeSigma™

ABT1487MI

--


Antigen:
alpha-Actin, Smooth Muscle

Classification:
Polyclonal

Conjugate:
Unconjugated

Gene:
ACTA2, AAT6, ACTSA, ACTVS, GIG46, MYMY5

Host Species:
Rabbit

Purification Method:
Affinity Purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
This polyclonal antibody targets the N-terminal sequence specific to smooth muscle alpha-actin (ACTA2), but not skeletal muscle alpha-actin (ACTA1), cytoplasmic beta-actin (ACTB), cardiac muscle alpha-actin (ACTC1), cytoplasmic gamma-actin (ACTG1), or smooth muscle gamma-actin (ACTG2; ACTA3).

Content And Storage:
Stable for 1 year at 2°-8°C from date of receipt.

Applications:
Immunohistochemistry, Immunoprecipitation, Western Blot

Concentration:
Please refer to lot specific datasheet.

Formulation:
Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.

Gene Accession No.:
P62736

Immunogen:
KLH-conjugated linear peptide corresponding to the N-terminual sequence of human smooth muscle alpha-actin.

Quantity:
100 μg

Research Discipline:
Cell Structure

Gene ID (Entrez):
NP_001135417

Target Species:
Human

Form:
__