Anti-TGF-beta Receptor Antibody, type I

Description

• General description: TGF-beta receptor type-1 (UniProt: P36897; also known as EC:2.7.11.30, TGFR-1, Activin A receptor type II-like protein kinase of 53kD, Activin receptor-like kinase 5, ALK-5, Serine/threonine-protein kinase receptor R4, SKR4, Transforming growth factor-beta receptor type I, TbetaR-I) is encoded by the TGFBR1 (also known as ALK5, SKR4) gene (Gene ID: 7046) in human. TGF-beta R1 is found in all tissues examined and is most abundant in placenta and least abundant in brain and heart. It is also expressed in a variety of cancer cell lines. TGF-beta signaling involves its binding to the TGF-beta receptor type II (TGF-beta RII), which allows it to recruit TGF-beta receptor type I (TGF-beta RI) and assemble it as a heterodimeric receptor complex. TGF-beta RII phosphorylates TGF-beta RI in the glycine-serine (GS) rich region and activates the serine/threonine kinase activity of TGF-beta RI, which in turn phosphorylates receptor-linked Smad (Small mothers against decapentaplegic) proteins. To prevent any spontaneous phosphorylation of Smads, the inhibitor molecule FKBP12 binds to the GS region of the TGF-beta RI and blocks the access of TGF-beta RII to this domain. This inhibitory effect is removed upon TGF-beta binding to the receptor.
• Specificity: This polyclonal antibody specifically detects TGF-beta receptor type 1 in multiple species. It targets an epitope of 15 amino acids in the C-terminal region.
• Immunogen: KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human TGF-beta receptor 1.
• Application: Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected TGF-beta receptor type-1 in human spleen, human cerebellum, human kidney, human pancreas, human placenta (chorionic villi), and human placenta (decidual cells).Flow Cytometry Analysis: 1 µg from a representative lot detected TGF-beta receptor type-1 in one million mouse splenocyte cells.
• Quality: Evaluated by Western Blotting in rat liver tissue lysate.Western Blotting Analysis: 1 µg/mL of this antibody detected TGF-beta receptor type-1 in 10 µg of rat liver tissue lysate.
• Target description: ~56 kDa observed, 55.96 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
• Linkage: Replaces: ABF17
• Other Notes: Concentration: Please refer to lot specific datasheet.