Monoclonal Anti-HLA Class I Antigen−FITC antibody produced in mouse

Description

• General description: Monoclonal Anti-Human HLA Class I Antigen (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cell line NS-1 and splenocytes from BALB/c mice immunized with human tonsil leukocyte membrane preparation. Class I proteins are highly polymorphic. HLA-A, -B, -C are classical class I proteins and are heterodimers. HLA-A consists of a polymorphic transmembrane α heavy chain and an invariant water-soluble light chain.
• Specificity: Recognizes the human HLA Class I antigen expressed on most human nucleated cells. The antibody reacts with a monomorphic epitope on the 44-45 kDa α-chain polypeptide of human class I HLA molecules (HLA-A,B,C). The antibody may be used in immunoprecipitation, complement mediated cytotoxicity, flow cytometry and immunohistochemical staining of acetone-fixed, frozen sections and cell smears. It cross-reacts with cells from African and Asian apes and old world monkeys. No reactivity is seen with cells from most new world monkeys or from non-primates. The epitope recognized by this antibody is sensitive to routine formalin fixation and paraffin embedding.4th Workshop: code no. P15th Workshop: code nos. BP166, BP288, BP407, B 005
• Immunogen: human tonsil leukocyte membrane preparation.
• Application: FITC Monoclonal Anti-Human HLA Class I Antigen may be used for immunochemical analysis of HLA Class I antigens; Studies of HLA-A,B,C expression on embryonic and neoplastic tissues; and/or the purification of HLA-A,B,C antigens.
• Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide
• Preparation Note: Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.
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