Milli-Mark Anti-CD3 -PE Antibody, clone UCHT1

Description

• General description: Human TCR/CD3 is a complex structure on the lymphocyte surface. It consists of the TCR alpha beta or TCR gamma delta heterodimer and the associated CD3 complex. The CD3 complex is composed of six polypeptides with usually four different transmembrane CD3 chains, gamma, delta, epsilon. and zeta. Three different dimers, gamma epsilon, delta epsilon, and zeta zeta, constitute the CD3 complex. The MW of CD3epsilon is 20 000 (Campana, 1987). CD3 is first detectable in early thymocytes and its appearance probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, during early stages of maturation, the CD3 antigen is predominantly present in the cell cytoplasm. In medullary thymocyles, the CD3 antigen is predominantly detected on the cell surface (Swerdlow, 1988; Beverley, 1981). The majority of T cell neoplasms also expresses CD3, but it is absent from non-T-cell lymphoid malignancies (McMichael, 1987). Consistent with the pattern of synthesis of the antigen in normal thymocytes, the earliest site detectable within neoplastic cells is the cell cytoplasm (Swerdlow, 1988).
• Specificity: Antibody recognizes Human CD3 antigen.
• Immunogen: Human infant thymocytes and lymphocytes from a patient with Sézary disease.
• Application: Milli-Mark Anti-CD3 -PE Antibody, clone UCHT1 is an antibody against CD3 for use in FC.
• Quality: Evaluated by flow cytometry using Human Lymphocytes.
• Target description: 20 kDa (Campana, 1987)
• Physical form: Protein A purified
• Storage and Stability: Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
• Analysis Note: ControlHuman Lymphocytes
• Other Notes: Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
• Legal Information: MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.