Milli-Mark® Anti-CD117 Antibody, c-kit-PE, clone 104D2

Description

• General description: CD117 is a 145 kDa transmembrane protein which belongs to the class III receptor tyrosine kinase family. The extracellular region consists of five immunoglobulin-like domains where the second and third loops are thought to be involved in ligand binding (Ashman, 1997; Bühring, 1995). CD117 has been shown to be a marker for tissue mast cells, hematopoietic stem cells, and progenitor cells in normal human bone marrow. The majority of CD117-positive marrow cells (50-70%) co-express CD34 and comprise progenitor cells and their precursors of all hematopoietic lineages. Apart from mast cells, CD117 is essentially absent from mature hematopoietic cells. Although CD117 is primarily a marker for non-lymphoid progenitor cells in the hematopoietic cells/tissues, including melanocytes, embryonic brain, and the reproductive system (Ashman, 1997). Anti-CD117, 104D2, was included in the Sixth International Workshop and Conference on Human Leucocyte differentiation Antigens, and studies by different laboratories confirmed its reactivity with CD117 (Ashman, 1997).
• Specificity: Antibody recognizes Human CD117 antigen.
• Immunogen: MOLM-1 megakaryocytic cell line.
• Application: Milli-Mark Anti-CD117 Antibody, c-kit-PE, clone 104D2 detects level of CD117 & has been published & validated for use in FC.
• Quality: Evaluated by flow cytometry using TF-1 cells
• Target description: 145 kDa (Ashman, 1997; Buhring, 1995)
• Physical form: Protein A purified
• Storage and Stability: Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt.
• Analysis Note: ControlTF-1 cells
• Legal Information: MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.