Monoclonal Anti-MDM2 antibody produced in mouse

Description

• General description: Mouse 3T3 cell double minute 2 (MDM2) gene was originally found on mouse double minute chromosomes.
• Specificity: The antibody shows predominantly nuclear and some cytoplasmic staining in cells.
• Immunogen: recombinant mouse MDM2.
• Biochem/physiol Actions: In addition, to regulation MDM2 also mediates the degradation of p53. MDM2 protein has been shown to activate cell proliferation by stimulating the S-phase-inducing transcription factors, E2F1/DP1. Overexpression of MDM2 results in reduced quantities of co-expressed p53, and disruption of the p53-MDM2 interaction by mutation results in both activation and accumulation of p53. The amplification of the MDM2 gene has been demonstrated in various human neoplasms. MDM2 is frequently overexpressed in the nuclei of soft-tissue sarcomas, and to a much lesser extent in a wide variety of malignancies, such as bladder, breast, testes, and cervix cancers, and in glial cells, and a range of hematological disorders. Monoclonal antibody reacting specifically with MDM2 is an essential tool in defining the interactions and distributions of MDM2, its function in signaling pathways and in mutual regulation of p53.
• Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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