Anti-Glutathione Peroxidase 4 Antibody, clone Mab63-1

Description

• General description: Phospholipid hydroperoxide glutathione peroxidase, cytosolic (UniProt: P36969-2; also known as PHGPx, Glutathione peroxidase 4, GPx-4, GSHPx-4) is encoded by the GPX4 gene (Gene ID: 2879) in human. Three functionally distinct isozymes of GPx4 have been described and are known as mGPx4 (mitochondrial), cGPx4 (cytosolic), and nGPx4 (nuclear). They originate from a single GPX4 gene. The cytosolic isoform is ubiquitously expressed at moderate levels in most mammalian cells. On the other hand, mitochondrial and nuclear isoforms are overexpressed in spermatoid cells. Clone Mab63-1 specifically recognizes cGPx4 in human and its ortholog in mouse. However, it does not recognize rat GPx4. GPx4 is a considered to be a moonlighting protein that has been implicated in anti-oxidative defense, gene expression regulation, and programmed cell death, and as a structural protein in spermatogenesis. Homozygous GPx4-deficient mice are reported to die utero before E7.5. Similarly, homozygous mice expressing catalytically inactive GPx4 variants (Sec46Ala/Ser mutant) do not survive beyond the 7th day of embryogenesis. (Ref.: Borchert, A., et al (2010). Immunol. Lett. 133 (2); 85-93).
• Specificity: Clone Mab63-1 specifically detects cytosolic Glutathione Peroxidase 4 in human and murine cells. it targets a minimum epitope of 6 amino acids from the N-terminal region.
• Immunogen: His-tagged purified recombinant human cytosolic glutathione peroxidase 4.
• Application: Anti-Glutathione Peroxidase 4, clone Mab63-1, Cat. No. MABF1969, is a mouse monoclonal antibody that detects cytosolic glutathione peroxidase 4 and has been tested for use in Dot Blot, Electron Microscopy, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
• Quality: Evaluated by Western Blotting in HEK293 cells transfected with Gpx4. Western Blotting Analysis: 2 µg/mL of this antibody detected Glutathione Peroxidase 4 in lysate of HEK293 cells transfected with Gpx4.
• Target description: ~19 kDa observed; 19.53 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
• Physical form: Protein G purified
• Storage and Stability: Stable for 1 year at 2-8°C from date of receipt.
• Other Notes: Concentration: Please refer to lot specific datasheet.
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.