Anti-Cytokeratin 19 Antibody, clone TROMA-3

Description

• General description: Keratin, type I cytoskeletal 19 (UniProt: P19001; also known as Cytokeratin-19, CK-19, Keratin-19, K19) is encoded by the Krt19 (also known as Krt1-19) gene (Gene ID: 16669) in murine species. CK-19 belongs to the family of intermediate filament (IF) family, but differs from other IF proteins in lacking the C-terminal tail domain. About 20 different cytokeratin proteins have been reported in higher animals in varying combinations during various stages of development. CK-19 is normally expressed in the lining of the gastroenteropancreatic and hepatobiliary tracts. It is expressed throughout embryonic development with highest levels observed at 8.5 days post coitum. Its expression is shown to decrease by day 11.5 and then is increased again by 17.5 days post coitum. CK-19 is shown to be involved in the organization of myofibrils and together with keratin 8, it helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. CK-19 has been shown to be an independent prognostic factor for pancreatic neuroendocrine tumors, especially the insulin-negative tumors. TROMA 3 has been found to stain trophoblastoma TDM.1 cells, a subpopulation of the retinoic acid.-induced F9 and PCC3/ND1 cells, and in 5-azacytidine-treated 1246 cells.
• Specificity: Clone TROMA-3 detects cytokeratin 19 in human and murine cells.
• Immunogen: Purified intermediate filaments from DM1 (Trophoblatoma) cell line.
• Application: Anti-Cytokeratin 19, clone TROMA-3, Cat. No. MABT913, is a rat monoclonal antibody that detects Keratin, type I cytoskeletal 19 and is tested for use in Electron Microscopy, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
• Quality: Evaluated by Western Blotting in MCF-7 cell lysate.Western Blotting Analysis: 0.5 µg/mL of this antibody detected Cytokeratin 19 in 10 µg of MCF-7 cell lysate.
• Target description: ~44 kDa observed; 44.54 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
• Physical form: Format: Purified
• Other Notes: Concentration: Please refer to lot specific datasheet.