Anti-N-Cadherin Antibody, clone 8C11

Description

• General description: Cadherin-2 (UniProt: P19022; also known as CDw325, Neural cadherin, N-cadherin, CD325) is encoded by the CDH2 (also known as CDNH, NCAD) gene (Gene ID: 1000) in human. N-Cadherin is a single-pass type I membrane glycoprotein protein that plays a role in calcium-dependent cell adhesion. Its expression is observed in cells of the nervous tissue, vascular smooth muscle cells, and myocytes. N-cadherins preferentially interacts with themselves in a homophilic manner in connecting cells; may thus contribute to the sorting of heterogeneous cell types. N-cadherin are synthesized as precursor proteins with a signal peptide (aa 1-25) and a propeptide (aa 26-159) that are proteolytically cleaved to generate functional, mature protein. Newly synthesized proN-cadherin (aa 1-906) is phosphorylated and proteolytically processed prior to transport to the plasma membrane. The mature N-Cadherin contains an extracellular domain (aa 160-724) with five homologous repeats that mediates adhesion, a single pass transmembrane domain (aa 725-745), and a conserved cytoplasmic domain (aa 746-906) that interacts with catenins to link cadherins to the actin cytoskeleton. N-cadherin is well expressed in endothelial cells and plays an essential role in the maturation and stabilization of normal vessels. However, it also promotes tumor cell survival, migration and invasion, and a high level of its expression is often associated with poor prognosis. A 69 amino acid segment of EC-4 of N-cadherin is reported to be essential and sufficient to increase cell motility and promote epithelial to mesenchymal transition. (Ref.: Kim, JB., et al. (2000). J. Cell Biol. 151(6); 1193-1205; Navarro, P., et al. (1998). J. Cell Biol. 140(6); 1475-1484).
• Specificity: Clone 8C11 is a mouse monoclonal antibody that detects N-Cadherin. It targets an epitope within the extracellular domain.
• Immunogen: MBP-tagged recombinant fragment corresponding to 502 amino acids from the extracellular domain of human N-Cadherin.
• Application: Quality Control TestingEvaluated by Western Blotting in Human heart tissue lysate.Western Blotting Analysis: A 1:500 dilution of this antibody detected Cadherin-2 in Human heart tissue lysate.Tested applicationsWestern Blotting Analysis: A 1:500 dilution from a representative lot detected N-Cadherin in Human brain and cerebellum tissue extracts.Western Blotting Analysis: A representative lot detected N-Cadherin in Western Blotting applications (Kim, J.B., et al. (2000). J Cell Biol. 151(6):1193-1206).Flow Cytometry Analysis: 1 μg from a representative lot detected N-Cadherin in one million KG-1 cells.Inhibition Assay: A representative lot inhibited motility of BT20 breast cancer cells (Kim, J.B., et al. (2000). J Cell Biol. 151(6):1193-1206). Flow Cytometry Analysis: A representative lot detected N-Cadherin in Flow Cytometry applications (Wahlig, S., et al. (2020). Sci Rep. 10(1):1681).Immunocytochemistry Analysis: A representative lot detected Cadherin-2 in Immunocytochemistry applications (Navarro, P., et al. (1998). J Cell Biol.;140(6):1475-1484).Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
• Physical form: Purified mouse monoclonal antibody IgG1 in PBS without preservatives.
• Storage and Stability: Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
• Other Notes: Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.