Anti-Platelet Factor 4 Antibody, clone 197.2

Description

• General description: Platelet factor 4 (UniProt P02776; also known as C-X-C motif chemokine 4, Iroplact, Oncostatin-A, PF-4) is encoded by the PF4 (also known as CXCL4, SCYB4) gene (Gene ID 5196) in human. Platelet factor 4 (PF4) is a small CXC chemokine family cytokine released from alpha-granules of activated platelets during platelet aggregation to promote blood coagulation. PF4 binds heparin with high affinity (Kd ~4-20 nM) via its heparin-binding domain (a.a. 92-98) and neutralizes the anti-thrombin activity of heparin-like molecules. PF4 is chemotactic for neutrophils, fibroblasts and monocytes, and interacts with the chemokine receptor CXCR3 splice variant CXCR3B. In individuals suffering from heparin-induced thrombocytopenia (HIT), PF4-heparin complexes can trigger an immune response and the generation of anti-PF4 antibodies, resulting in the foramtion of macromolecular antibody-heparin-PF4 immune complexes that bind Fc RIIa (CD32a) on platelets and induce platelet activation. Platelet activation in turn results in more PF4 release into circulation, enhanced immune-complex formation, exaggerated platelet activation, and cell clearance. PF4 is synthesized with a signal peptide sequence (a.a. 1-31) that is removed posttranslationally to produce the mature PF4 (a.a. 32-101), additional proteolytic cleavage yields the PF4 short form (a.a. 48-101).
• Specificity: Clone 197.2 targets a linear epitope sensitive to heparin binding, but insensitive to reducing agent or denaturation by SDS (Xiao, Z., et al. (2008). Blood. 112(4):1091-1100).
• Immunogen: Purified human platelet PF4 (Xiao, Z., et al. (2008). Blood. 112(4):1091-1100).
• Application: Detect PF4 using this mouse monoclonal Anti-Platelet Factor 4, clone 197.2, Cat. No. MABS1255, validated for use in ELISA, Flow Cytometry, Function Analysis, Immunocytochemistry. and Western Blotting.
• Quality: Evaluated by Flow Cytometry in human platelets.Flow Cytometry Analysis: 1 µg of this antibody detected PF4 in one million 4% paraformaldehyde-fixed, 0.2% Triton X-100-permeabilized human platelets.
• Target description: ~8 kDa (recombinant mature hPF4) observed. 10.84 kDa (pro-form), 7.769 kDa (mature; a.a. 32-101), 6.033 kDa (cleaved short form; a.a. 48-101) calculated. Uncharacterized bands may be observed in some lysate(s).
• Physical form: Format: Purified
• Other Notes: Concentration: Please refer to lot specific datasheet.
• Legal Information: ALEXA FLUOR is a trademark of Life Technologies