MABS835

Anti-EGFR Antibody, clone 7F12.1

clone 7F12.1, from mouse

Manufacturer: Sigma Aldrich

Synonym(S): Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

Select a Size

Pack Size SKU Availability Price
50 μG MABS835-50-μG In Stock ₹ 42,360.00

MABS835 - 50 μG

₹ 42,360.00

In Stock

Quantity

1

Base Price: ₹ 42,360.00

GST (18%): ₹ 7,624.80

Total Price: ₹ 49,984.80

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

7F12.1, monoclonal

species reactivity

human

technique(s)

flow cytometry: suitableimmunocytochemistry: suitablewestern blot: suitable

isotype

IgG2bκ

NCBI accession no.

NP_005219

UniProt accession no.

P00533

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Description

  • General description: Epidermal growth factor receptor (EC 2.7.10.1; UniProt P00533; also known as Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1) is encoded by the EGFR (also known as ERBB, ERBB1, HER1, NISBD2) gene (Gene ID 1956) in human. The epidermal growth factor receptor (EGFR; Proto-oncogene c-ErbB-1) is a ubiquitously receptor tyrosine kinase that is activated by a variety of ligands. Upon ligand-bound EGFR undergoes dimerization and autophosphorylation exposing cytoplasmic domains, which allows its interaction with adaptor proteins and induction of several signaling pathways including PI3K-AKT; PLC-gamma-PKC; Notch1; and Ras-Raf. Hence, it contributes to a diverse range of cellular processes, including cell proliferation. Several studies have reported that EGFR signaling plays an important role in the development of various tumors. EGFR is regulated by PTPRJ and PTPRK phosphatases, and by the endocytic machinery including EPS15 that mediates internalization and degradation of the receptor.
  • Specificity: Epitope region is present in spliced isoform 1 (p170), but not in isoform 2 (p60, Truncated, TEGFR), isoform 3 (p110) or isoform 4 reported by UniProt (P00533).
  • Immunogen: GST-tagged recombinant human EGFR fragment correspoding to a cytoplasmic region C-terminal to the kinase domain.
  • Application: Detect EGFR using this mouse monoclonal , Cat. No. MABS835, validated for use in Flow Cytometry, Immunocytochemistry, and Western Blotting.
  • Quality: Evaluated by Western Blotting in A431 cell lysate.Western Blotting Analysis: 0.1 µg/mL of this antibody detected EGFR in 10 µg of A431 cell lysate.
  • Target description: ~170 kDa observed. Target band size appears larger than the calculated molecualar weight of 134.3 kDa due to glycosylation. Uncharacterized bands may be observed in some lysate(s).
  • Linkage: Replaces: 04-338
  • Physical form: Format: Purified
  • Storage and Stability: Stable for 1 year at 2-8°C from date of receipt.
  • Other Notes: Concentration: Please refer to lot specific datasheet.
  • Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

SAFETY INFORMATION

WGK

WGK 1

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mouse

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primary antibodies

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7F12.1, monoclonal

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human

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flow cytometry: suitableimmunocytochemistry: suitablewestern blot: suitable

isotype:
IgG2bκ

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NP_005219

UniProt accession no.:
P00533

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