Anti-Bovine IgM (μ-chain specific) antibody produced in rabbit

Description

• General description: During the B-cell development, IgM (immunoglobulin M) is the first expressed antibody. It is present as a monomer on the surface of naive B cells. Antigenic stimulation and upon maturation, the monomeric IgM units (190kDa), are linked by disulfide bonds to form a pentameric immunoglobulin structure. It also but rarely forms a hexameric structure. The pentameric IgM is linked by a disulfide bond, a polypeptide chain and a J-chain. The μ -chain consists of five domains- VH, Cμ1, Cμ2, Cμ3 and Cμ4.
• Specificity: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against Bovine IgM and Bovine Serum. No reaction was observed against Bovine IgG F(ab′)2 or Bovine IgG F(c).
• Immunogen: Bovine IgM mu heavy chain
• Biochem/physiol Actions: The J-chain of the multimeric IgM (immunoglobulin M) structure is responsible for secretion at the mucosal surfaces. The pentameric form of IgM, has a greater affinity towards antigens (with multiple repeating epitopes) compared to the monomers. IgM pentamer enables better opsonization of antigens for destruction and fixing complement.
• Physical properties: Antibody format: Antiserum
• Physical form: Supplied in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Reconstitution: Reconstitute with 1.0 mL deionized water (or equivalent).
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