Anti-Centrin Antibody, clone 20H5 ZooMAb® Mouse Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 20H5 is a ZooMAb® mouse recombinant monoclonal antibody that specifically detects centrin (Caltractin). It targets an epitope within the C-terminal region.
• Immunogen: Recombinant Chalamydomonas reinhardtii centrin.
• Application: Quality Control TestingEvaluated by Western Blotting in Mouse testis tissue lysate.Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Centrin in Mouse testis tissue lysate.Tested ApplicationsWestern Blotting Analysis: A 1:10,000 dilution from a representative lot detected Centrin in human testis tissue lysate.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Centrin in human testis tissue sections.Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Centrin in human testis tissue sections.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
• Target description: Caltractin (UniProt: P05434; also known as 20 kDa calcium-binding protein, Centrin) is encoded by the VFL2 gene (Gene ID: 5725094) in Chlamydomonas reinhardtii (algae). Centrin is a highly conserved calmodulin-like calcium-binding phospho-protein that contain four EF hands and are associated with centrosome-related structures. In Chlamydomonas reinhardtii, centrin is shown to play an essential role in microtubule severing during flagellar excision during which time stellate fibers of the transition zone contract and displace the microtubule doublets of the axoneme inward. The resulting shear force and torsional load generated during inward displacement leads to microtubule severing immediately distal to the central cylinder of the transition zone. Centrin is also present is all eukaryotic cells and contain four helix-loop-helix motifs that all conform to the EF-hand consensus for Ca2+ binding. Two of these motifs exhibit high affinity for Ca2+ two display lower affinity. Stability of centrin is regulated by phosphorylation of serine residue in the C-terminal region by aurora kinase A, which also localizes with centrin at the centrosome. Their levels are shown to be the highest from prophase to metaphase stage followed by precipitous loss in the late stage of mitosis. In human breast cancers higher levels of aurora kinase A and phospho-centrin have been observed. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Sanders, MA., and Salisbury, JL. (1994). J. Cell Biol. 24(5); 795-805; Lutz, W., et al. (2001). J. Biol. Chem. 276(23); 20774-20780; Lukasiewicz, KB., et al. (2011). PLoS ONE 6(6); e21291).
• Physical form: Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.