Anti-PNPT1 Antibody, clone 2D23 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 2D23 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects PNPase 1 (PNPT1). It targets an epitope within 20 amino acids from the C-terminal region.
• Immunogen: KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of human PNPase 1 (PNPT1).
• Application: Quality Control TestingWestern Blotting Analysis: A 1:1,000 dilution of this antibody detected PNPT1 in HeLa cell lysate.Tested applicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected PNPT1 in Jurkat, NIH3T3, and C6 cell lysates.Affinity Binding Assay: A representative lot of this antibody bound PNPT1 peptide with a KD of 2.5 x 10-8 in an affinity binding assay.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected PNPT1 in human stomach tissue sections.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
• Target description: Polyribonucleotide nucleotidyltransferase 1, mitochondrial (UniProt: A8TCS8; also known as EC:2.7.7.8, 3′-5′ RNA exonuclease OLD35, PNPase old-35, Polynucleotide phosphorylase 1, PNPase 1, Polynucleotide phosphorylase-like protein, PNPT1) is encoded by the PNPT1 (also known as PNPASE) gene (Gene ID: 87178) in human. PNPT1 is a peripheral homotrimer membrane protein that serves as a RNA-binding protein that is implicated in several RNA metabolic processes. It catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3′-to-5′ direction. It is synthesized with a transit peptide (aa 1-45) and is localized to mitochondrial intermembrane region. It is a component of the mitochondrial degradosome (mtEXO) complex with exoribonuclease activity that degrades 3′ overhang double-stranded RNA with a 3′-to-5′ directionality in an ATP-dependent manner. It is reported to regulate the expression of the electron transport chain components at the mRNA and protein levels. PNPT1 is shown to be essential for correct processing and polyadenylation of mitochondrial mRNAs and plays a role as a cytoplasmic RNA import factor that mediates the translocation of small RNA components into the mitochondrial matrix. It is also reported to participate in RNA cell surveillance by cleaning up oxidized RNAs. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.