Anti-MRGPRX4 Antibody, clone 3F4 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 3F4 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Mas-related G-protein coupled receptor member X4 (MRGPRX4). It targets an epitope within 16 amino acids from the C-terminal, cytoplasmic domain.
• Immunogen: KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal, cytoplasmic domain of human Mas-related G-protein coupled receptor member X4 (MRGPRX4).
• Application: Quality Control TestingEvaluated by Immunohistochemistry (Paraffin) in rat spinal cord tissue sections.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected MRGPRX4 in rat spinal cord tissue sections.Tested ApplicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected MRGPRX4 in lysates from Human Dorsal Root Ganglia, Human Spinal Cord, and U-87 and U251 cells.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MRGPRX4 in SH-SY5Y cells.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
• Target description: Mas-related G-protein coupled receptor member X4 (UniProt: Q96LA9; also known as Sensory neuron-specific G-protein coupled receptor 5/6, MRGPRX4) is encoded by the MRGPRX4 (also known as MRGX4, SNSR5, SNSR6) gene (Gene ID: 117196) in human. MRGPRX4 is a multi-pass membrane glycoprotein with four extracellular domains, seven transmembrane domains, and four cytoplasmic domains. It is shown to be localized in a subset of small dorsal root and trigeminal sensory neurons. It has been classified as an orphan receptor that is involved in the regulation of nociceptor function and/or development, including the sensation or modulation of pain. It is shown to be potently activated by enkephalins. Several unconjugated and conjugated bile acids are reported to act as ligands for MRGPRX4 and may contribute to cholestatic pruritus. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Meixiong, J., et al. (2019). Proc. Natl. Acad. Sci. USA. 116(21); 10525-10530; Yu, H., et al. (2019). eLife. 8; e48431).
• Physical form: Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.