Anti-TSC2 Antibody, clone 4H10 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 4H10 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Tuberin (TSC2). It targets an epitope within 24 amino acids from the C-terminal region.
• Immunogen: KLH-conjugated linear peptide corresponding to 24 amino acids from the C-terminal region of human Tuberin.
• Application: Quality Control TestingWestern Blotting Analysis: A 1:1,000 dilution of this antibody detected TSC2 in SH-SY5Y cell lysate.Tested applicationsAffinity Binding Assay: A representative lot of this antibody bound TSC2 peptide with a KD of 2.7 x 10-7 in an affinity binding assay.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected TSC2 in human kidney tissue sections.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected TSC2 in SH-SY5Y cell line.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
• Target description: Tuberin (UniProt: P49815; also known as Tuberous sclerosis 2 protein, TSC2) is encoded by the TSC2 (also known as TSC4) gene (Gene ID: 7249) in human. TSC2 is a peripheral membrane protein that serves as a tumor suppressor and is expressed in multiple tissues. In complex with TSC1 it inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. It acts as a GTPase-activating protein (GAP) for the small GTPase Rheb, a direct activator of the protein kinase activity of mTORC1. In its C-terminal region it contains a highly conserved Rap-Gap domain (aa 1531-1758) that is essential for its function. TSC2 has been reported to increase the intrinsic GTPase activity of both Rap1A and Rab5 in vitro. TSC2 can undergo phosphorylation at serine 1387, 1418 or 1420. However, these phosphorylations do not affect its interaction with TSC1. It can also undergo phosphorylation at serine 939 and threonine 1462 by Akt1 induced by growth factor stimulation. When phosphorylated and activated by AMPK under conditions of low ATP:AMP ratio, it leads to negative regulation of the mTORC1 complex. AMPK-mediated phosphorylation leads to TSC1-TSC2 complex stabilization, increasing GAP activity toward Rheb and allows turning off mTORC1 signaling. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Li, Y., et al. (2004). Trends Biochem. Sci. 29(1); 32-38).
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.