Anti-MR-proADM Antibody, clone 2B19 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 2B19 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects human ADM. It targets an epitope within the mid-region (MR) of ADM.
• Immunogen: KLH-conjugated linear peptide corresponding to 24 amino acids from the propeptide N-terminal half of human ADM.
• Application: Quality Control TestingEvaluated by Western Blotting with recombinant Human ADM Protein.Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant human ADM protein.Tested ApplicationsImmunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MR-proADM in human placenta tissue sections.Affinity Binding Assay: A representative lot of this antibody bound MR-proADM peptide with a KD of 9.9 x 10-7 in an affinity binding assay.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
• Target description: pro-ADM (UniProt: P35318; also known as Mid-regional pro-adrenomedullin) is encoded by the ADM (also known as AM) gene (Gene ID: 133) in human. ADM is synthesized with a signal peptide (aa 1-21) and is cleaved into Adrenomedullin (AM; aa 95-146) and Proadrenomedullin N-20 terminal peptide (PAMP; aa 21-41). AM and PAMP serve as potent hypotensive and vasodilatory agents involved in physiologic control of fluid and electrolyte homeostasis. In the kidney, AM acts as a diuretic and natriuretic, and both AM and PAMP are known to inhibit aldosterone secretion. ADM contains a region known as mid-region ProADM (aa 45-92) that lacks any activity but is a reliable biomarker for sepsis and progression to septic shock. ADM has been found in all epithelial surfaces that separate the internal and external environment, such as integument, respiratory, digestive and genito-urinary tract. It is also found in all bodily secretions and imparts protection against external pathogens. Its highest levels are reported in pheochromocytoma and adrenal medulla. ADM as well as PAMP also display potent antimicrobial action against both Gram-positive and Gram-negative bacteria. MR-proADM, a fragment of 48 amino acids that splits from the proADM and is present in a ratio of 1:1 with ADM, proportionally represents the levels and activity of ADM. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Onal, U., et al. (2018). Healthcare (Basel). 6(3); 110; Valenzuela-Sanchez, F., et al. (2016). Ann. Transl. Med. 4(17); 329).
• Physical form: Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.