Anti-eIF3h Antibody, clone 2B15 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 2B15 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Eukaryotic translation initiation factor 3 subunit H (elF3h). It targets an epitope within 21 amino acids from the C-terminal region.
• Immunogen: KLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal region of human Eukaryotic translation initiation factor 3 subunit H (elF3h).
• Application: Quality Control TestingEvaluated by Western Blotting in HEK293 cell lysate.Western Blotting Analysis: A 1:10,000 dilution of this antibody detected eIF3h in HEK293 cell lysate.Tested ApplicationsWestern Blotting Analysis: A 1:10,000 dilution from a representative lot detected eIF3h in A431, PC12, and NIH3T3 cell lysates.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected eIF3h in human pancreas tissue sections.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected eIF3h in A431 and HepG2 cells.Affinity Binding Assay: A representative lot of this antibody bound eIF3H peptide with a KD of 1.1 x 10-6 in an Affinity Binding Assay,Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
• Target description: Eukaryotic translation initiation factor 3 subunit H (UniProt: O15372; also known as eIF3h, Eukaryotic translation initiation factor 3 subunit 3, eIF-3-gamma, eIF3 p40 subunit) is encoded by the EIF3H (also known as EIF3S3) gene (Gene ID: 8667) in human. elF3h is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex that is required for several steps in the initiation of protein synthesis. The elF3 complex is composed of 13 different subunits that are divided into three modules. elF3h belongs to module B that also contains elF3f and elf3M. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex is reported to specifically target and initiate translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
• Physical form: Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.