Anti-GAD67 Antibody, clone 4B5 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 4B5 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Glutamate decarboxylase 1 (GAD67). It targets an epitope within 20 amino acids from the N-terminal region.
• Immunogen: KLH-conjugated linear peptide corresponding to 20 amino acids from the N-terminal region of human Glutamate decarboxylase 1 (GAD67).
• Application: Quality Control TestingEvaluated by Western Blotting in Rat Brain tissue lysate.Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GAD67 in Rat brain tissue lysate.Tested ApplicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected GAD67 in Human brain tissue lysate.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected GAD67 in human cerebral cortex tissue sections.Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected GAD67 in human cerebral cortex tissue sections.Affinity Binding Assay: A representative lot of this antibody bound GAD67 peptide with a KD of 2.3 x 10-8 in an affinity binding assay.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
• Target description: Glutamate decarboxylase 1 (UniProt: Q99259; also known as EC:4.1.1.15, 67 kDa glutamic acid decarboxylase, GAD-67, Glutamate decarboxylase 67 kDa isoform) is encoded by the GAD1 (also known as GAD, GAD67) gene (Gene ID:2571) in human. GAD67 is responsible for the conversion of glutamic acid to gamma-aminobutyric acid (GABA), the major inhibitory transmitter in higher brain regions, and putative paracrine hormone in pancreatic islets. Two molecular forms of GAD have been described (65 kDa and 67 kDa) that display 64% amino acid homology and both forms are expressed in the CNS, pancreatic islet cells, testis, oviduct, and ovary. Between the two isoforms, GAD67 is responsible for over 90% of basal GABA synthesis and is produced at limiting levels in the brain. These isoforms are regionally distributed in cytoplasm in the brains of rats and mice. GAD67 expression is shown to change during neural development in rat spinal cord and its expression seems to be particularly plastic and can change in response to experimental manipulation and disease progression. Mutations in GAD1 gene are known to cause spastic quadriplegic cerebral palsy, a non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
• Physical form: Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.