Anti-Aggrecan Antibody, clone 4B10 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 4B10 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Aggrecan. It targets an epitope within 21 amino acids from the N-terminal region.
• Immunogen: KLH-conjugated linear peptide corresponding to 21 amino acids from the N-terminal region of human Aggrecan.
• Application: Quality Control TestingWestern Blotting Analysis: A 1:1,000 dilution of this antibody detected Aggrecan in Human Chondrocytes.Tested applicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected Aggrecan in Human Mesenchymal Stem Cells.Affinity Binding Assay: A representative lot of this antibody bound Aggrecan peptide with a KD of 2.4 x 10-8 in an affinity binding assay.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Aggrecan in human cartilage tissue sections.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Aggrecan in human chondrocytes.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
• Target description: Aggrecan core protein (UniProt: P16112; also known as Cartilage-specific proteoglycan core protein, CSPCP, Chondroitin sulfate proteoglycan core protein 1, Chondroitin sulfate proteoglycan 1) is encoded by the ACAN (also known as AGC1, CSPG1, MSK16) gene (Gene ID: 176) in human. Aggrecan, a secreted proteoglycan, is a major component of extracellular matrix of cartilaginous tissues. Its major function is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region and may play a regulatory role in the matrix assembly of the cartilage. It is synthesized with a signal peptide (aa 1-16), which is subsequently cleaved off to generate the mature aggrecan core protein. Aggrecan contains two globular domains, G1 and G2 that comprise the N-terminus of the proteoglycan, while another globular region, G3, makes up the C-terminus. The G1 region is located in the N-terminus of the core protein and interacts with hyaluronic acid (HA). G1 contains Link domains and thus consists of three disulfide-bonded loop structures designated as the A, B, B′ motifs. The G2 region is composed of two B-like domains, but does not have the ability to interact with HA. G2 region is separated from the G1 region by the interglobular domain, which is a prominent site for proteolysis. The keratan sulfate (KS) and the chondroitin sulfate (CS) attachment domains lie between G2 and G3. Aggrecan is present in the extracellular matrix in the form of proteoglycan aggregates, in which many aggrecan molecules interact with hyaluronan and a link protein stabilizes each interaction. It is reported that aggrecan structure changes throughout the life cycle due to changes in synthetic and degradative events. Changes due to synthesis alter the structure of the chondroitin sulfate and keratan sulfate chains, whereas those due to degradation cause cleavage of all components of the aggregate. Aggrecan is also present in most of the retina including the outer nuclear layer, the outer plexiform layer, and the inner plexiform layer. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.