Anti-LC3A Antibody, clone 3J12 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 3J12 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Autophagy-related protein LC3A. It targets an epitope within 25 amino acids from the N-terminal region.
• Immunogen: KLH-conjugated linear peptide corresponding to 25 amino acids from the N-terminal region of human LC3A.
• Application: Quality Control TestingWestern Blotting Analysis: A 1:10,000 dilution of this antibody detected LC3A in Rat Brain tissue lysate.Tested applicationsWestern Blotting Analysis: A 1:10,000 dilution from a representative lot detected LC3A in Human brain, mouse brain and PC12 lysates.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected LC3A in SH-SY5Y cells.Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected LC3A in human cerebral cortex tissue sections.Affinity Binding Assay: A representative lot of this antibody bound LC3A peptide with a KD of 3.3 x 10-8 in an affinity binding assay.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
• Target description: Microtubule-associated proteins 1A/1B light chain 3A (UniProt: Q9H492; also known as Autophagy-related protein LC3A, Autophagy-related ubiquitin-like modifier LC3A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, MAP1A/MAP1B LC3 A, Microtubule-associated protein 1 light chain 3 alpha) is encoded by the MAP1LC3A gene (Gene ID: 84557) in human. LC3s are structural proteins of autophagosomal membranes and are widely used as biomarkers of autophagy. They are ubiquitously distributed in all eukaryotes and are expressed as three splice variants known as LC3A, LC3B, and LC3C. LC3A is mainly localized to perinuclear and nuclear regions, LC3B is evenly distributed throughout the cytoplasm and is also localized in the nucleolar regions, and LC3C is shown to be located in the cytoplasm and strongly in the nuclei. Two variants (v1 and v2) of LC3A have been described that have different transcriptional start sites. Nuclear LC3A is shown to co-localize with Beclin-1. All three LC3s show a conserved region that is required for autophagosome formation. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Koukourakis, MI., et al. (2015). PLoS ONE 10(9); e0137675).
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.