Anti-γ-Actin Antibody, clone 6J13 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 6J13 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects g-Actin. It targets an epitope within 13 amino acids from the N-terminal.
• Immunogen: KLH-conjugated linear peptide corresponding to 13 amino acids from the N-terminal region of human g-Actin.
• Application: Quality Control TestingEvaluated by Western Blotting in HepG2 cell lysate.Western Blotting Analysis: A 1:1,000 dilution of this antibody detected y-Actin in HepG2 cell lysate.Tested ApplicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected y-Actin in HeLa, C6, and mouse kidney tissue lysates.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected g-Actin in human small intestine tissue sections.Flow Cytometry Analysis: 0.1 μg from a representative lot detected g-Actin in one million HeLa cells.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected g-Actin in HepG2 cells.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
• Target description: Actin, cytoplasmic 2 (UniProt: P63261; also known as Gamma-actin) is encoded by the ACTG1 (also known as ACTG) gene (Gene ID: 71) in human. Actins are ubiquitous eukaryotic proteins that serve as multi-functional, basic building blocks of cytoskeletal microfilaments. They play critical roles in a wide range of cellular processes, including cell division, cell migration, chromatin remodeling, transcriptional regulation, and vesicle trafficking. These functions are attributed to their ability to form filaments, which can quickly assemble and disassemble depending upon the needs of the cell. At least six different actin types have been reported in mammals. Although actins show about 90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expression patterns and only 50-60% homology is found in their 18 N-terminal residues. -actin is a cytoplasmic actin that is highly conserved in higher animals and is predominantly expressed in non-muscle cells where it controls cell structure and motility. Unlike β-actin that is enriched at the leading edge of migrating cells, -actin is more uniformly distributed throughout the cell. -actin knockout is reported to cause developmental delays, reduced postnatal and adult survival, and progressive myopathy. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Belyantseva, EA., et al. (2009). Proc. Natl. Acad. Sci. USA. 106(24); 9703-9708).
• Physical form: Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.