Anti-alpha Actinin 2 Antibody, clone 1E11, ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 1E11 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Alpha-Actinin-2. It targets an epitope within 17 amino acids from the N-terminal region.
• Immunogen: KLH-conjugated linear peptide corresponding to the first 17 amino acids from the N-terminal region of Alpha-actinin-2 from human skeletal muscle.
• Application: Anti-alpha Actinin 2, clone 1E11 ZooMAb , Cat. No. ZRB1169, is a highly specific rabbit recombinant monoclonal antibody that targets alpha-Actinin-2 and has been tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
• Target description: Alpha-actinin-2 (UniProt: P35609; also known as Alpha-actinin skeletal muscle isoform 2, F-actin cross-linking protein) is encoded by the ACTN2 gene (Gene ID: 88) in human. Alpha-actinin-2 is expressed in both skeletal and cardiac muscle. It is considered as a bundling protein that acts to crosslink filamentous actin molecules and titin molecules from adjoining sarcomeres at Z-discs, a function that is modulated by phospholipids. It has a rod-shaped structure and it homodimerizes in an anti-parallel fashion. Each monomer contains an N-terminal actin-binding region composed of two calponin homology domains (aa 38-142 and 151-257), two C-terminal EF hand domains (aa 753-788 and 789-824), and four tandem spectrin-like repeats (aa 281-391, 401-506, 516-627, and 637-740). It has also been shown to localize to dendritic spines, enriched within the post-synaptic density (PSD). In rat hippocampal neurons loss of alpha-actinin-2 is shown to create an increased density of immature, filopodia-like protrusions that fail to mature into a mushroom-shaped spine during development and its knockdown prevents the recruitment and stabilization of PSD in the spine, resulting in failure of synapse formation. Mutations in ACTN2 gene is known to cause cardiomyopathy with ventricular hypertrophy leading to dyspnea, syncope, collapse, palpitations, and chest pain. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Hodges, JL, et al. (2014). PLoS ONE 9(7): e101770).
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.