Anti-Recoverin Antibody, clone 1J23, ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 1J23 ZooMAb is a rabbit recombinant monoclonal antibody that specifically detects human Recoverin.
• Immunogen: His-tagged recombinant full-length human Recoverin.
• Application: Anti-Recoverin, clone 1J23 ZooMAb , Cat. No. ZRB1107, is a recombinant rabbit monoclonal antibody that specifically targets Recoverin and has been tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
• Target description: Recoverin (UniProt: P35243; also known as Cancer-associated retinopathy protein, Protein CAR) is encoded by the RCVRN (also known s RCV1) gene (Gene ID: 5957) in human. Recoverin is a member of the EF-hand neuronal calcium sensor (NCS) proteins that is expressed in retinal photoreceptors and midget cone bipolar cells. It unctions as a calcium sensor that regulates rhodopsin phosphorylation through inhibition of rhodopsin kinase. It contains four EF hand motifs (aa 25-60; 61-96; 97-132; 147-182) and a myristoyl chain on the N-terminus. It has a high affinity (aa 110-121) and a low affinity (aa 74-85) calcium binding site. Recoverin undergoes a conformational change upon calcium binding in darkness and subsequently it attaches to the disc membrane in photoreceptor outer segment. This in turn allows binding of G protein-coupled receptor kinases (GRK). Calcium-bound Recoverin is shown to inhibit G-protein-coupled receptor kinase (GRKs) in darkness, which prevents visual pigment phosphorylation. Under bright light condition, calcium dissociates from Recoverin and the protein translocates to the inner segment. The released GRK can phosphorylate and deactivate the light activated visual pigment. Mutations in Recoverin have been linked to retinal dysfunction associated with early-stage diabetes and cancer-associated retinopathy. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Zang. J., and Neuhauss, SCF (2018). Front. Mol. Neurosci 11:473).
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Stable for 1 year at 2-8°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.