Anti-Desmin Antibody, clone 1H16, ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 1H16 is a ZooMAb , rabbit recombinant monoclonal antibody that specifically detects Desmin. It targets an epitope within the C-terminal region.
• Immunogen: GST/His-tagged recombinant fragment corresponding to 64 amino acids from the C-terminal region of human Desmin.
• Application: Anti-Desmin, clone 1H16 ZooMAb , Cat. No. ZRB1178, is a highly specific rabbit recombinant monoclonal antibody that targets Desmin and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
• Target description: Desmin (UniProt: P17661) is encoded by the DES (also known as CDCD3, CMD1F, CSM1) gene (Gene ID: 1674) in human. Desmin is a homopolymeric protein that is classified as a muscle-specific type III intermediate filament. It is shown to be essential for proper muscular structure and function. It plays a crucial role in maintaining the structure of sarcomeres, inter-connecting the Z-disks and forming the myofibrils, linking them not only to the sarcolemmal cytoskeleton, but also to the nucleus and mitochondria, thus providing strength for the muscle fiber during activity. It also acts as a sarcomeric microtubule-anchoring protein that specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction. Desmin can undergo phosphorylation at multiple serines by CDK1. Phosphorylation at serine 60 by Aurora B and threonine 76 by ROCK1 is reported to contribute to efficient separation of Desmin intermediate filaments during mitosis. Mutations in DES gene have been linked to myofibrillar myopathy that is characterized at ultrastructural level by disintegration of the sarcomeric Z disk and myofibrils, and replacement of the normal myofibrillar markings by small dense granules, or larger hyaline masses, or amorphous material. It leads to skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, and restrictive heart failure. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.