Anti-Iba1/AIF1 Antibody, clone 16B14, ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 16B14 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Allograft inflammatory factor 1 (AIF1). It targets an epitope within 21 amino acids from the C-terminal region.
• Immunogen: KLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal region of human Allograft inflammatory factor 1 (AIF1).
• Application: Anti-Iba1/AIF1, clone 16B14 ZooMAb, Cat. No. ZRB1177, is a highly specific recombinant rabbit monoclonal antibody that detects Iba1/AIF1 and has been tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
• Target description: Allograft inflammatory factor 1 (UniProt: P55008; also known as AIF1, Ionized calcium-binding adapter molecule 1, Protein G1) is encoded by the AIF1 (also known as G1, IBA1) gene (Gene ID: 199) in human. AIF1/IBA1 is a highly conserved cytoplasmic, calcium binding, inflammation responsive scaffold protein that is detected in T-lymphocytes and peripheral blood mononuclear cells. It has also been shown to associate with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis. It is not present in normal arteries, but its expression is induced in vascular smooth muscle cells in response to arterial injury where it enhances growth and promote proliferation in vascular smooth muscle cells through deregulation of the cell cycle by shortening the cell cycle and altering the expression of cyclins. AIF1 is also found in activated macrophages where its expression is augmented by cytokines, particularly interferon gamma. It is also considered as a marker for activated macrophages. Serum AIF-1 levels have been correlated with albuminuria and eGFR in type 2 diabetic subjects and serves as a marker for diabetic nephropathy. Three isoforms of AIF1/IBA1 have been described that are produced by alternative splicing. This clone will react with isoforms 1 and 2 that contain this immunogen sequence. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. Fukui, M., et al. (2012). Diabetes Res. Clin. Pract. 98(1); 146-150; Autieri, MV., and Carbone, CM (2001). Arterioscler. Thromb. Vasc. Biol. 21(9);1421-1426.
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 300 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.